diversity of cells Flashcards
histology
study of tissue at a microscopic leve
histological technique
tissue processing, embedding, sectioning and staining
tissue processing
tissue is fixed to preserve it in a life like state. To do this you must dehydrate then replace the water in tissue with alcohol then replace with xylene and then with wax
embedding and sectioning
Thin sections are cut on a microtome and put onto microscopic glass slides, the wax is washed out and the tissue rehydrated. Next we want to embed the tissue we have just processed into a wax block
staining
Staining is a process by which a tissue section is colored and are of three main types.
acidic stains
The acidic stains are negatively charged and combine with positively charged particles. They are red or pink in colour, Eosin is a commonly used acidic stain. The tissue components that stain with acidic dye are termed acidophilic (Red, pink).
basic stains
Basic stains are the opposite They are positively charged, and combine with negatively charged particles They are blue or dark purple in color, Hematoxylin & toluidine blue are the commonly used ones. Tissue components that stain with basic dye are termed basophilic (Blue).
neutral stains
The neutral stains combine with both positively and negatively charged particles and give different colour to different components.
4 basic tissue types
epithelium, connective, muscle, nervous. all organs are made up of a combination of these
epithelial
covers surfaces of the body, line hollow organs, form glands
characteristics of epithelial tissue
The first one is polarity of the cells, such as apical and basal. the cells of epithelial tissue tightly adhere to each other by the means of intercellular junctions and cytoskeletal elements. Its also avascular which means there are no blood vesicles
classifications of epithelial tissue
glandular and covering
glandular
Exocrine- has ducts to transport secretion. Ducts lined w simple cuboidal
Endocrine – lack duct system. Secrete into blood stream
covering
simple- one layer. Shapes = squamous, cuboidal, columnar
Stratified- multiple layers. Shapes = “ “ and transitional Pseudo stratified- one layer looks stratified
surface specialisation
in covering epithelium. some types have special characteristics to carry out jobs
cilia
hair like texture, moves things eg. mucus from trachea
keratin
dead cells, protection eg skin