diversity of cells Flashcards

1
Q

histology

A

study of tissue at a microscopic leve

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2
Q

histological technique

A

tissue processing, embedding, sectioning and staining

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3
Q

tissue processing

A

tissue is fixed to preserve it in a life like state. To do this you must dehydrate then replace the water in tissue with alcohol then replace with xylene and then with wax

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4
Q

embedding and sectioning

A

Thin sections are cut on a microtome and put onto microscopic glass slides, the wax is washed out and the tissue rehydrated. Next we want to embed the tissue we have just processed into a wax block

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5
Q

staining

A

Staining is a process by which a tissue section is colored and are of three main types.

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6
Q

acidic stains

A

The acidic stains are negatively charged and combine with positively charged particles. They are red or pink in colour, Eosin is a commonly used acidic stain. The tissue components that stain with acidic dye are termed acidophilic (Red, pink).

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7
Q

basic stains

A

Basic stains are the opposite They are positively charged, and combine with negatively charged particles They are blue or dark purple in color, Hematoxylin & toluidine blue are the commonly used ones. Tissue components that stain with basic dye are termed basophilic (Blue).

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8
Q

neutral stains

A

The neutral stains combine with both positively and negatively charged particles and give different colour to different components.

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9
Q

4 basic tissue types

A

epithelium, connective, muscle, nervous. all organs are made up of a combination of these

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10
Q

epithelial

A

covers surfaces of the body, line hollow organs, form glands

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11
Q

characteristics of epithelial tissue

A

The first one is polarity of the cells, such as apical and basal. the cells of epithelial tissue tightly adhere to each other by the means of intercellular junctions and cytoskeletal elements. Its also avascular which means there are no blood vesicles

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12
Q

classifications of epithelial tissue

A

glandular and covering

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13
Q

glandular

A

Exocrine- has ducts to transport secretion. Ducts lined w simple cuboidal
Endocrine – lack duct system. Secrete into blood stream

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14
Q

covering

A

simple- one layer. Shapes = squamous, cuboidal, columnar
Stratified- multiple layers. Shapes = “ “ and transitional Pseudo stratified- one layer looks stratified

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15
Q

surface specialisation

A

in covering epithelium. some types have special characteristics to carry out jobs

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16
Q

cilia

A

hair like texture, moves things eg. mucus from trachea

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17
Q

keratin

A

dead cells, protection eg skin

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18
Q

microvilli

A

thick brush bristles, absorption eg small intestine

19
Q

goblet cells

A

produce mucus

20
Q

connective tissue

A

forms framework of the body, dynamic role in development, growth and homeostasis of tissue and in energy store (via fat)

21
Q

types of connective

A

soft, hard, dense

22
Q

soft connective

A

tendons, ligaments, mesentery, stroma of organs and skin

23
Q

hard

A

bone and cartiliage

24
Q

dense

A

packed bundles of collagen fibres

25
Q

cartilage

A

strong, flexible. Semi rigid nature comes from highly hydrated nature of ground substance. Its avascular, receives nutrients from adjacent tissues by diffusion through matrix.

26
Q

types of cartilage

A

hyaline, fibrocartilage, elastic

27
Q

hyaline

A

most common, clear matrix

28
Q

fibrocartilage

A

collagen fibre bundles, single chondrocytes

29
Q

elastic

A

thin elastic fibres, groups of chondrocytes

30
Q

bones cell types

A

osteocytes, osteoclasts, osteoblasts

31
Q

types of bone

A

compact and spongy

32
Q

osteons

A

in compact bone, aligned in same direction, prevent bone resist bending or breaking

33
Q

muscle

A

specialised to contract and generate force, cells filled w contractile fibres actin and myosin.

34
Q

types of muscle tissue

A

cardiac, skeletal, smooth

35
Q

smooth muscle

A

elongated, central nucleus, longitudinal and transverse sections

36
Q

cardiac

A

has striations, short branching fibres, single central nucleus, intercalated discs (sites of end to end attachments between branching cells, intercellular junctions), forms major part of walls of the heart and great vessels

37
Q

skeletal

A

striations, non branching fibres, multinucleated cells, peripheral nucleus

38
Q

nervous tissue

A

Specialised to conduct impulses. Consists of neurons and supporting cells. Connective tissue coat (meninges and perineuium)

39
Q

type of neurons

A

multipolar, bipolar, pseudo-unipolar

40
Q

multipolar

A

multiple dendrites fibres one axon

41
Q

bipolar

A

one dentrite, one axon, can both branch.

42
Q

pseudo-unipolar

A

one cytoplasmic process

43
Q

supporting cells

A

microglia, astrocytes, oligodendrocytes. M provide immune surveillance. O wrap around axon to myelinate it. Schwann cell can also myelinate