Detection of Antibodies Flashcards
Antibodies exhibit weak reactivity at high dilutions; dissociate easily due to low avidity
High-Titer Low-Avidity
Antibodies with titers usually ≥1:64, often over 1:1000, react weakly but can appear clinically significant
High-Titer Antibodies
Lab screening detects antibodies in donor plasma; discard plasma if antibodies are seen
Donor Lab Screening
Antibody identification in patients to determine compatible blood or glucose level
Px Lab Screening
Detection of clinically significant alloantibodies that cause decreased RBC survival, usually IgG antibodies that react at 37°C
Clinically Significant Alloantibodies
Detect antibodies by reacting patient serum with reagent RBCs phenotyped for multiple antigens like C, c, D, E, Fya, Fyb, Jka, Jkb, K, k, Lea, Leb, P1, M, N, S, s
Antibody Detection and Identification
Reagent RBCs used in antibody detection to identify clinically significant antibodies while minimizing the detection of insignificant ones
Reagent RBCs
Antigram result to identify antibodies; positive test indicates the need for antibody identification, negative result eliminates positive agglutination
Antigram
IgG antibodies that cause decreased RBC survival, typically reacting at 37°C.
Clinically Significant Alloantibodies
Antibody Detection and Identification involves reaction of
patient serum with reagent RBCs phenotyped for multiple antigens
O cells phenotyped for multiple antigens used in antibody detection and identification.
Reagent RBC
Detect clinically significant antibodies, minimize detection of insignificant antibodies, and complete the procedure in a timely manner.
Purpose of Antibody Identification
Positive result indicates need for antibody identification; negative result eliminates positive agglutination.
Antigram
Purpose of Antibody Identification
Detect significant antibodies, minimize insignificant ones, and complete efficiently.
Antigram results interpretation
Positive results needs antibody identification; negative result rules out agglutination.
