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BLOODBANK/IMMUNOHEMATOLOGY > Component Preparation > Flashcards

Component Preparation Flashcards

1
Q

Time for whole blood collection (450-500 mL).

A

8 to 12 minutes.

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2
Q

Time to reject blood unit if clotting occurs during collection.

A

12 minutes or above.

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3
Q

Temperature for blood bank refrigerator storage.

A

1-6°C (QC: 1.5 to 5.5°C).

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4
Q

Temperature for storage of platelet concentrate.

A

Room temperature (20-24°C) with continuous agitation.

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5
Q

Storage temperature for plasma-containing components.

A

Frozen at -20°C or colder.

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6
Q

Time for soft spin centrifugation.

A

3000/3200 rpm for 2-3 minutes.

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7
Q

Time for hard spin centrifugation.

A

3500-3600 rpm for 5 minutes.

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8
Q

Storage period for blood components with AS-1, AS-3, AS-5, AS-7 additives.

A

42 days.

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9
Q

Rejuvenation solution used for RBC regeneration in the US.

A

Rejuvesol.

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10
Q

Time to submit whole blood collected at 1-6°C.

A

Within 24 hours.

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11
Q

Time to submit whole blood collected at 20-24°C.

A

Within 6 hours.

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12
Q

Temperature for RBC components during transport.

A

Wet ice.

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13
Q

Transport requirement for platelet and WBC components.

A

Without ice.

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14
Q

Time for processing and centrifugation of whole blood for PRP and RBC components.

A

2 to 3 minutes (soft spin).

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15
Q

Time for platelet poor plasma centrifugation.

A

5 minutes (hard spin).

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16
Q

Needle size for blood collection.

A

16 gauge needle.

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17
Q

Blood storage requirement for red blood cells post-transfusion.

A

More than 75% RBC survival within 24 hours.

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18
Q

Allergy to iodine: alternative antiseptic.

A

Chlorhexidine gluconate solution.

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19
Q

Allergy to iodine and chlorhexidine: alternative antiseptic.

A

70% isopropyl alcohol.

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20
Q

Time for rejuvenation of RBCs with solution after outdating.

A

1-4 hours at 37°C.

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21
Q

Fresh Whole Blood volume for preparation.

A

450-500 cc

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22
Q

Platelet Rich Plasma volume for preparation.

A

200-250 cc

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23
Q

Platelet Poor Plasma volume for preparation.

A

200 cc

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24
Q

Platelet Concentrate volume for preparation.

A

50-70 cc

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25
Q

PRBC volume for preparation.

A

200-250 cc

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26
Q

FFP volume for preparation.

A

200 cc

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27
Q

Cryosupernate volume for preparation.

A

180-200 cc

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28
Q

Cryoprecipitate volume for preparation.

A

15-20 cc

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29
Q

Storage condition for Platelet Concentrate.

A

Room temperature with agitation (3-5 days)

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30
Q

Storage condition for Fresh Frozen Plasma.

A

Freezer temperature

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31
Q

Components in Cryoprecipitate.

A

FVIIIc, FI (fibrinogen), FXIII, vWF, fibronectin

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32
Q

Storage condition for Cryoprecipitate.

A

Freezer temperature

33
Q

Components in Cryosupernate.

A

Same as FFP but decreased in FVIIIc, FI, FXIII, vWF, fibronectin

34
Q

Procedure for Cryoprecipitate preparation.

A

Slow cold thawing process of Fresh Frozen Plasma

35
Q

Platelet agitation duration for storage.

A

3-5 days

36
Q

Method for separating Platelet Rich Plasma.

A

Soft spin

37
Q

Method for separating Platelet Poor Plasma and Platelet Concentrate.

A

Hard spin

38
Q

Method of Leukocyte Removal.

A

Centrifugation, Washing procedures (using saline or glycerol), Mechanical separation using leukoreduction filters

39
Q

First generation leukoreduction filters size.

A

170 um

40
Q

Second generation leukoreduction filters size.

A

20-40 um

41
Q

Third generation leukoreduction filters size; removes 99.9% of WBC.

A

3-log filter

42
Q

Components able to pass through leukoreduction filters: standard filter of 170-200 um.

A

Normal platelets, normal RBCs

43
Q

Components removed by (microaggregate) leukoreduction filters; 20-40um.

A

Fibrin clots, giant platelets, macrocytes, large RBCs, large WBC fragments, most white blood cells

44
Q

Leukoreduction Definition.

A

Removal of white blood cells (WBCs) from blood or blood components prior to transfusion

45
Q

Residual WBCs per each whole blood or red blood cells after leukoreduction.

A

<5 x 10^6 WBCs

46
Q

Residual WBCs per each platelet derived from whole blood after leukoreduction.

A

<8.3 x 10^5 WBCs

47
Q

Recovery of original component after leukoreduction.

A

> 85% of original component must be recovered

48
Q

Leukoreduction Categories - Pre-storage.

A

Performed shortly after collection

49
Q

Leukoreduction Categories - Post-storage.

A

At the patient’s bedside

50
Q

Leukoreduction Filters Usage.

A

Microaggregate filter or leukoreduction filter

51
Q

Device designed to count WBCs at exceptionally low levels for leukoreduction quality control.

A

Nagoette Chamber

52
Q

Instrument used in leukoreduction quality control to count WBCs at low levels.

A

Flow Cytometer

53
Q

Purpose of irradiation in transfusion?

A

Prevents transfusion-associated GVHD by inactivating T cells; expires in 28 days.

54
Q

Minimum gamma radiation dose?

A

25 Gy (central); 15 Gy (any part).

55
Q

Radioactive sources for irradiation?

A

Cesium-137 or Cobalt-60.

56
Q

Irradiation quality control method?

A

Radiographic film label darkening.

57
Q

Purpose of washing blood components?

A

Removes extracellular solution to prevent FNHTR, TRALI, and allergic reactions.

58
Q

Storage for washed RBCs?

A

1–°6C, up to 24 hours.

59
Q

Storage for washed platelets?

A

20–24°C, transfuse within 4 hours.

60
Q

Indications for washing?

A

Allergic reactions, antibody, and substance removal.

61
Q

Purpose of pooling blood components

A

Allows multiple blood components to be transfused in a single event.

62
Q

Pooling of multiple RBC units

A

Not typically performed; other product types may be pooled.

63
Q

Reconstituted whole blood composition

A

Type O RBC + AB plasma; used for neonatal exchange transfusions.

64
Q

Expiration time for pooled products

A

4 hours.

65
Q

Maximum storage duration for frozen RBCs

A

10 years.

66
Q

Cryoprotective agents in RBC freezing

A

Penetrating (e.g., dimethylsulfoxide) and nonpenetrating (e.g., hydroxyethyl starch) agents.

67
Q

Initial freezing temperature for high glycerol RBCs

A

-80°C.

68
Q

Freezer type for high glycerol RBCs

A

Mechanical freezer.

69
Q

Maximum storage temperature for high glycerol RBCs

A

-65°C.

70
Q

Shipping requirements for high glycerol RBCs

A

Dry ice with 40% glycerol.

71
Q

Freezing temperature for low glycerol RBCs

A

-196°C in liquid nitrogen.

72
Q

Storage temperature for low glycerol RBCs

A

-120°C in liquid nitrogen (20% glycerol).

73
Q

Saline concentrations used in deglycerolization

A

First: 12% saline; Second: 1.6% saline; Third: 0.09% saline with 0.2% dextrose.

74
Q

Adjustment for sickle cell trait donors during deglycerolization

A

1.6% saline step is omitted.

75
Q

Method for glycerol removal in a closed system

A

Automated removal of glycerol.

76
Q

Expiration of deglycerolized RBCs in AS-3 at 4°C (automated closed system)

A

14 days.

77
Q

Advantages of RBC freezing

A

Long-term storage, maintenance of RBC viability and function, low residual leukocytes and platelets, removal of significant plasma proteins.

78
Q

Disadvantages of RBC freezing

A

Time-consuming process, higher cost of equipment and materials, storage requirements (-65°C), higher cost of products.

BLOODBANK/IMMUNOHEMATOLOGY (22 decks)

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