CMA CH 30 Key Terms - Basic Microbiology Flashcards
aerobic
organism that requires oxygen for survival and growth.
aerosols
particles from potentially infectious materials that could be released into the air
agar
gelatin-line substance extracted from red algae that contains nutrients and moisture for bacteria growth.
anaerobic
organism that needs little to no oxygen for survival and growth
broth tubes
tubes filled with broth substance that will support the growth of certain microorganisms
expectorate
act of coughing up material from airways that lead to the lungs
genus
first Greek or Latin name given to a microorganism; always capitalized.
Gram stain
named for inventor, Hans Christian Gram. Most common stain used in microbiology to observe gross morphologic features of bacteria; a differential stain, allowing differentiation between Gram-negative and Gram-positive organisms.
incubate
allowing a pathogen to grow in culture media
inoculate
to place colonies of microorganisms onto nutrient media for culture testing.
morphology
form and structure of an organism
mycology
study of fungi
nomenclature
naming
normal flora
microorganisms that are normally present in a specific site
nosocomial
infection acquired in a health care setting
ova
eggs; in a laboratory setting usually refers to eggs of a parasite
potassium hydroxide
10% solution placed on vaginal smears, as well as skin scrapings, hair, and other dry substances, to dissolve excess debris. This clears the vision field better for viewing of fungi and spores.
species
second Greek or Latin name given to microorganisms; not capitalized.
spores
inactive state of some bacteria in which they are encapsulated in protein. The encapsulation protects them from heat, chemicals, freezing, desiccation, and radiation and antibiotics. Spores can live for thousands of years with no nutrients. When spores are exposed to fertile human tissue they can become activated and grow. Tetanus is one type of bacteria that can create spores.
virology
study of viruses.
wet mount
method of adding liquid, usually saline or potassium hydrochloride, to a specimen on a slide for examination and preservation. The specimen is placed on a slide and one drop of saline (for dx of trichomonas vaginalis) or potassium hydroxide (for dx of vaginal yeast infections) is applied and mixes with the specimen. It is then covered with a coverslip and examined microscopically.
Wood’s Lamp
special light used to detect organisms that fluoresce such as certain fungi, bacteria, and parasites. Scabies and ringworm are two examples. Scratches in the eye may be detected using a Wood’s lamp after te eye has been stained with a fluorescent dye. Also used in determining margin dissection of melanoma.