Chapter 13 Vocab Flashcards
Antiparallel
Referring to the arrangement of the sugar phosphate backbones in a DNA double helix (thy run in opposite 5’ to 3’ directions)
Bacteriophage
A virus that infects bacteria, also called a phage
Chromatin
the complex of DNA and proteins that makes up eukaryotic chromosomes. When the cell is not dividing, chromatin exists in its dispersed form, as a mass of very long, thin fibers that are not visible with a light microscope
DNA Ligase
A linking enzyme essential for DNA replication, catalyzes the covalent bonding of the 3’ end of one DNA fragment to the 5’ end of another DNA fragment
DNA Polmymerase
An enzyme that catalyzes the elongation of new DNA by the addition of nucleotides to the 3’ end of an existing chain. There are several different DNA polymerase, DNA polymerase 3 and DNA polymerase 1 play major roles in DNA replication in E. Colu
DNA Replication
The process by which a DNA molecule is copied, also called DNA synthesis
Double helix
The form of native DNA, referring to its 2 adjacent antiparallel polynucleotide strands wound around an imaginary axis into a spiral shape
Helicase
An enzyme that untwist the double helix of DNA at replication forks, separating the two strands and making them available as template strands
Lagging strand
A discontinuously synthesized DNA strand that elongates by means of Okazaki fragments, each synthesized in a 5 to 3 direaction awasy from the replication fork
Leading strand
The new complementary DNA strand synthesized continuously along the template strand toward the replication fork in the mandatory 5 to 3 direction
Mismatch repair
The cellular process that uses specific enzymes to remove and replace incorrectly paired nucleotides
Nuclease
An enzyme that cuts DNA or RNA, either removing one or a few bases or hydrolizing he DNA or RNA completely into its component nucleotides
Nucleoid
A nonmembrane enclosed region in a prokaryotic cell where it’s chromosome is located
Nucleotide excision repair
A repair system that removes and then correctly replaces a damaged segment of DNA using the undamaged strand as a guide
Okazaki fragment
A short segment of DNA synthesized awasy from the replication fork on a template strand during DNA replication. Many such segments are joined together to make up the lagging strand of newly synthesized DNA