chapter 1 molecular biology

Question 1

hybridoma cell lines

Answer 1

produce monoclonal antibodies

Question 2

antibodies

Answer 2

can be used to detect specific molecules

Question 3

colormetric

Answer 3

can be used to support flourescnece in an argument basis

Question 4

hemotoxolin and eosin

Answer 4

stains tissue but it is a dye

Question 5

immunoflourescance

Answer 5

stains tissue with antibodies

Question 6

colormetric

Answer 6

probe to stain, tissue is also an antibody

Question 7

primary antibody

Answer 7

can bind to anything

Question 8

western blotting or immunoblotting

Answer 8

proteins transferred gel electrophoresis and probed with a specific antibody

Question 9

CDNA library

Answer 9

complementary DNA library
-it represent DNA with only genes that are expressed, no introns

Question 10

GDNA library

Answer 10

genomic DNA library
-represent entire genome (exons and introns included)

Question 11

DNA library

Answer 11

petri dish with DNA that represents genome of specific organism (cut long DNA into smaller fragments)

Question 12

PCR

Answer 12

isolate a couple of cells, isolate DNA and amplify it to make more, one cell pcr can make as much as you want
-in theory you can just keep replicating it

Question 13

In Situ Hybridixation

Answer 13

can reveal the location of specific mrna
-the probe is the mrna not the antibody
-binds to the RNA and DNA and creates a color reaction