Chapter 1 Intro Flashcards
Do microbes and micro-organisms mean the same thing?
Yes
Where are microbes found?
Everywhere on earth that supports life
Are most microbes harmless or helpful?
Yes, others can cause disease
Microbes live in complex microbial -
Communities with one another
Micro-organisms can be-
Cultivated (Produced)
What are the main types of micro-organisms?
Bacteria, Archaea, Protozoa, Algae, Fungi, Viruses, Helminths
Microbes can be grown on-
Solid or liquid nutrient media
On solid media, a single cell can become millions of cells that can be seen with the unaided eye. This is called-
A Colony
Is being able to grow microbes essential to their study?
Yeah
What are the various roles played by microbes?
Human Health + Animal Health, Ecosystem Health + Natural Resources, Bioenergy + Biotechnology, Agriculture + Food, Industry, Water & Waste
The human body has how many body cells?
30 Trillion
The human body has how many bacterial cells?
40 Trillion
Microbes that live stably on the human body =
The Human Microbiome
Microbes that live stably on the human body can be called the human microbiome. What other things can they be called?
Normal Microbiota or Normal Flora
Normal flora can aid in-
Digestion
Aside from digestion, normal flora can aid in-
Protection from invading microbes that can cause disease
Invading microbes that can cause disease =
Pathogens
When do we begin acquiring microbiota?
Before Birth
When do we begin acquiring viruses, fungi, and bacteria?
After Birth
The fundamental unit of life =
Cells
What states that cells are the fundamental unit of life?
The Cell Theory
Living entities surrounded by a membrane that are capable of growing, reproducing, metabolizing, and evolving =
Cells
Cells are capable of-
Growing, reproducing, metabolizing, and evolving
Do cells come in a variety of sizes?
Yup
Smallest living things =
Single-Celled Organisms
Groups of cells comprise-
Multi-Cellular Organisms
Cells can be described as-
Prokaryotic or Eukaryotic
Do prokaryotic cells lack a nucleus, or have a nucleus?
They lack a nucleus
Do eukaryotic cells lack a nucleus, or have a nucleus?
They have a nucleus
What kinds of cells lack various internal structures bound with phospholipid membranes?
Prokaryotic
Typically, how big are prokaryotic cells in diameter?
1 μm in diameter
Bacteria and Archaea are both what kinds of cells?
Prokaryotic
What kind’s of cells have a nucleus?
Eukaryotic Cells
How big are Eukaryotic Cells?
10 μm in diameter
What kind of cells have a more complex structure?
Eukaryotic Cells
Eukaryotic Cells include-
Algae, Protozoa, Fungi, Helminths, Animal Cells, and Plant Cells
How big are Animal Cells?
~10 μm in diameter
How big are bacteria?
~1 μm in diameter
How big are viruses?
100x smaller than bacteria (Nanometer Range)
What category of microscope is required to view viruses, bacteria, and eukaryotic cells?
Light Microscopes (Light Microscopy)
What type of microscope is commonly used in a wide variety of laboratory applications as the standard microscope; produces an image on a bright background?
Brightfield Microscopes
What type of microscope increases contrast without staining by producing a bright image on a darker background; especially useful for viewing live specimens?
Darkfield Microscopes
What type of microscope uses refraction and interference caused by structures in the specimen to create high contrast, high resolution images without staining, making it useful for viewing live specimens, and structures such as endospores and organelles?
Phase Contrast Microscopes
What type of microscope uses interference patterns between different patterns to enhance contrast between different features of a specimen to produce high-contrast images of living organisms with a three-dimensional appearance, making it especially useful in distinguishing structures within live, unstained specimens; images viewed reveal detailed structures within cells?
Differential Interference Contrast (DIC) Microscopes
What type of microscope uses fluorescent stains to produce stains to produce an image; can be used to identify patterns, to find particular species to distinguish living from dead cells, or to find locations of particular molecules within a cell; also used for immunofluorescence?
Fluorescence Microscopes
What category of microscope uses visible or ultraviolet light to produce an image?
Light Microscopy
Light Microscopes have a magnification of up to about-
1000x
Name off the types of microscopes that fall into the light microscopy category
Brightfield, Darkfield, Phase Contrast, DIC, Fluorescence
Name off the category of microscope that uses electron beams focused with magnets to produce an image
Electron Microscopy
Electron Microscopes have a magnification of-
20-100,000x or more
What are the types of microscopes that make up the electron microscopy category of microscopes?
Transmission (TEM)
Scanning (SEM)
What type of microscope uses electron beams that pass through a specimen to visualize small images; useful to observe small, thin specimens such as tissue sections and sub-cellular structures?
Transmission (TEM)
What type of microscope uses electron beams to visualize surfaces; useful to observe the three-dimensional surface details of specimens?
Scanning (SEM)
3 Domains of Life =
Archaea + Bacteria + Eukarya
Are there microbes from all 3 domains of life?
Yes
Bacteria + Archaea are both what kinds of microbes?
Prokaryotic Microbes
Why are Bacteria and Archaea both considered to be Prokaryotic Microbes?
Because their genetic material is not contained within a nucleus
Pathogens can cause disease in-
Humans + Plants + Animals
Most bacteria are harmless or beneficial.
True or false?
True
Bacteria is found nearly everywhere on Earth.
True or false?
True
Most Bacteria contain a unique cell wall component called-
Peptidoglycan
Bacteria are often described in terms of their-
Shape
Coccus =
Round Bacteria