Chap. 6 (Vcab.) Flashcards
Light Microscope (LM)
Visible light passes through specimen then through lenses, which refract light in a way that specimen is magnefied
Magnification
Ratio of an objects image size to its real size
Resolution
Measure of clarity of image
Contrast
Difference in brightness between light + dark areas of an image
Brightfield
Light passes directly through the specimen
Phase-contrast
Uses light to make unstained, transparent, and colorless specimens visible
Differential interference contrast (Nomarski)
Used to exaggerate differences in density; the image appears almost 3-D
Fluorescence
Uses a special microscope to view objects that emit fluorescent light
Confocal
Laser creates a single plane of fluorescence; out-of-focus light from other planes is eliminated.
Standard Fluorescence
Micrograph is blurry because out-of-focus light is not excluded
Deconvolution
Computer improves quality of images from microscope by increasing resolution + contrast
Super-resolution
Fluorescence microscopy technique that images cellular structures in more detail
Scanning Electron Microscope (SEM)
- Micro. that uses electron beam to scan surface of sample, coated with metal atoms
- Study detail of cell surfaces
Transmission Electron Microscope (TEM)
- Passes electron beam through thin sections stained with metal atoms
- Used to study internal structure of cells
Organelle
Membrane-enclosed structures with specialized functions
Electron Microscope (EM)
- Micro. that uses magnets to focus an electron beam on/through specimen
- 100-fold greater than light micro.
Cell Fractionation
Technique used to separate different components of a cell from each other by disrupting the cell + centrifugating mixture at diff. speeds
Differential Centrifugation
Centrifugate spins test tubes with mixtures of disrupted cells at increasing speeds
Pellet
Force from centrifugation causes cell components to settle at the bottom
Plasma Membrane
Barrier that separates cells interior from exterior environment
Cystosol
Semifluid, jellylike substance, portion of cytoplasm
Chromosomes
Carry genes in form of DNA
Ribosomes
Make proteins according to instructions of genes
Nucleoid
- Non-membrane enclosed region where DNA (chromosomes) is located
- Prokaryote
Cytoplasma
- Gel-like substance that fills inside of cell, where most of cells reactions occur
- In E cells, is region between plasma membrane and nucleus
Plasma Membrane
Membrane outside of cell that is selective, regulating the cell’s chemical composition
Nucleus
Contains genetic material in form of chromosomes
Nuclear Envelope
Double membrane that surrounds nucleus, separating contents from cytoplasma
Nuclear Lamina/ Intermidiate filaments
Protein filaments that line inner surface on nuclear envelope, maintain shape
Pore Complex
Protein structure that lines pores + regulates entry and exit of protein + RNAs
Chromosome
Carries genes made of DNA
Chromatin
Complex of DNA + proteins inside chromosome
Nucleolus
Structure in nucleus containing rRNA