🍃Biology RPs Flashcards
1
Q
Bio: what is the process for the microbiology required practical
A
- Clean your hands and workspace, using disinfectant spray to clean the worktop, wipe it off with a paper towel
- Use a permanent maker to mark black underneath the agar plate (with bacteria) making sure that the lid does not come off, so the bottom of the container looks as the image below
- Wash your hands with antibacterial wash, place 3 discs of antiseptic on the plate using forceps with the plate angled away from your face, record the antiseptic in each section
- Secure the lid with 2 small pieces of clear tape but do not make it airtight
- Incubated the plate at 25 degrees for 48 hours and record how far the bacteria were removed from the place of the antiseptic
2
Q
Bio: what is the process for the microscopy required practical
A
- Place the slide on the microscope and position the smallest objective lens so that it is almost touching the slide
- Increase the distance between slide and objectives lens using the course adjustment knob while looking through the eyepiece until the subject comes into focus
- Use teg fine adjustment knob to bring the cells into sharp focus
- Switch to a higher power objective lens and focus on teh cells in the same way, then take a detailed drawing for the cells, put the magnification underneath and label it
3
Q
Bio: what is the process for the osmosis required practical
A
- Use a cork borer to take 5 potato cylinders of the same diameter and use a knife to trim any skin at the end of each cylinder
- Accuratly measure and record the mass and length of each cylinder
- Measure 10cm3 of each concentration of sugar solution into 5 boiling tubes and respectively add a label to them with their concentrations, add one piece of potato to each of the boiling tubes
- Leave each piece of potato in the boiling tubes for the same length of time
- Remove the potato pieces, blot them dry with paper towel and measure and record the new mass and lengths, make a table of results
4
Q
Bio: what is the process for the food test for sugar required practical
A
- Set up a water bath
- Put some food sample into a test tube and add a few drops of Benedict’s solution
- Put the test tube in the water bath at 80 degrees for a minimum of 5 minutes and note down any colour change
5
Q
Bio: what is the method for the food test for starch required practical
A
- Put buffer solutions into test tubes (PH2,4,7,9,11)
- Place into water bath at roughly 35 degrees
- Add starch solution
- Set up spotting tile with iodine in dimples (2-3 drops)
- Add amylase to first test tube
- Start timer every 30 seconds take 2-3 drops of solution in pippette
- Place into spotting tile
- Repeat with other test tubes when previous has gone orange in spotting tile
6
Q
Bio: what is the process for the food test for lipids required practical
A
- Put some sample of food and a few drops of distilled water into a test tube
- Add a few drops of ethanol
- Shake gently
- Not any change
7
Q
Bio: what is the process for the food test for protein required practical
A
- Put some of eth sample into the test tube and then add 1 cm3 of biuret solution, but be careful because it is toxic and corrosive
- Shake gently
- Note any change
8
Q
Bio: what are the simple steps for the microscopy required pracical
A
- Focus on the sample with the smallest objective lens
- Re-focus with the larger
- Make a drawing
9
Q
Bio: what is the process for the enzymes required practical
A
- Heat the water bath to 35 degrees and then add 2 cm3 of buffered solution (check if this is right) to different test tubes and label each one with the correct pH
- Label test tubes “starch” and measure 4 cm3 of starch solution into each and put a thermometer in oven of them
- Add 10 cm3 of analyze to another test tube and label it
- Put all test tubes into the water bath and allow the solutions to reach 35 degrees
- Put one drop of iodine solution into each dimple on the spotting tray while you wait for the test tubes to warm. Also place some starch solution into the first dimple as a zero time, iodine goes blue black when starch is present and stays brown when no starch is present
- When the tubes have reached 35 degrees take one of the starch solution tubes out and add 2 cm3 of analyze solution using a pipette and add it to the test tube
- As soon as the analyze is put in start the stopwatch and stir the mixture with a glass rod
- After 10 seconds remove a drop and add it to the iodine
- Rinse the glass rod after 10 seconds, take another drop and add it to the iodine, repeat until there is no change in the iodine, record the pH and time taken for it to happen.
10
Q
bio: what is the process for the photosynthesis required practical
A
- Put a 10cm piece of pondweed with the cut end facing up in a beaker of water and cover the pondweed with an inverted filter funnel, see the image below
- Une a ruler to position the pondweed 1 meter away from the light source
- Turn on the light, start the stopwatch and record the number of bubbles released in 3 minutes, record the results
- Move the light source 20 cm closer and repeat the experiment
11
Q
Bio: what is the process for the reaction time required practical
A
- Person one sits with forearm on the table and looks at the 1cm ruler, it is held by person slightly through person ones hand with the 0cm mark at the top of the persons hand
- Person 2 drops at a random time the ruler and person 1 catches the ruler as soon as possible record the results using a conversion table to find the reaction time
- Repeat the test with person 1 enough times to take an average and then repeat with person 2
12
Q
Bio: what is the process for the plant response required practical
A
- Set up 3 petri dishes containing wetted wool and put 10 mustard seeds in each
- Put the dishes in a warm place and allow the seeds to germinate, water as needed
- Measure the height of each seed and then place one petri dish in each, direct sunlight, partial light and one in darkness
- Measure the height of each seedling each day for 5 days and record results in a table
13
Q
Bio: decay rp
A
