Biology Lab Exam 1 Flashcards
What is the Celsius equation?
(F-32)/1.8
What is the Fahrenheit equation?
(Celcius*1.8)+32
What is the scientific method?
- make an observation
- ask a question
- make an educated guess(hypothesis)
- test the hypothesis (experiment)
- obtain results
- draw a conclusion
What is a better buffer? Distilled water or sodium bicarbonate?
Sodium bicarbonate
What has a higher rate of change in pH? Distilled water vs. buffer solution?
Rate of change in h2o»rate of change w/ buffer
What is Brownian motion?
Random movement of particles
Method for lugol’s iodine(starch)?
- Test tubes (water and starch)
- 3 drops of lugol’s iodine and check for color
- Yellow/red is negative and blue-black is positive
What is the method for Benedict’s test(sugar)?
- Test tubes(water and sugar)
- 3 drops of Benedict’s reagent to each and mix
- Boil for 3 minutes
- Light blue is negative green/yellow/red/orange is positive
What is the method for paper spot test(lipid)?
- Two pieces of paper bag
- One drop of water and oil separately to each
- Let dry
- Stain is lipid and clear is no lipid
What is method for Sudan IV for lipids?
- Test tubes (one water and one oil)
- 1 squirt of water to both
- 6 drops of Sudan 4 to both tt
- 10 drops of water to water tt
- 10 drops of oil to oil tt
- Let stand for 6 minutes
- Uniform pink is negative and split pink is positive
What is the method for buiret test for protein
- Two test tubes one with water and one with protein
- 1 squirt of water to water and 1 squirt of albumin to protein
- 10 drops of biuret and mix
- Light blue is negative and pink/purple is positive
Why do plant cells have chloroplasts?
This is where photosynthesis takes place
What do animal cells have?
No cell wall and lysosomes
What do plant cells have?
Chloroplasts and vacuoles
What do bacteria cells have?
No nucleus, membrane bound organelles, and small/simple
