Biochem - lab techniques Flashcards
1
Q
PCR
A
denature, anneal premade primers, cool, elongate w/heat-stable DNA Pol
2
Q
southern blot
A
DNA chopped up and searched for specific sequences
3
Q
northern blot
A
RNA chopped up and searched for specific sequences
- good for levels of transcription and gene expression
4
Q
western blot
A
protein run and labeled antibodies used to search for peptides
5
Q
southwestern blot
A
ID’s DNA-binding proteins using labeled oligonucleotide probes
6
Q
Enzyme-Linked ImmunoSorbent Assay (ELISA)
- 2 types: differentiate
A
- indirect: looks for antibody using an antigen
2. direct: looks for antigen using an antibody
7
Q
FISH
A
probes bind to genes on chromosomes
localizes genes
8
Q
RNAi
A
dsRNA synthesized complementary to target mRNA
when introduced into cell, will separate, latch onto target and knock it out
- reduces specific gene expression
