ATP dependent pumps and ion exchangers

Question 1

What is key role of na-K ATPase pump?

Answer 1

set up concentration gradients to drive secondary active transport

Question 2

Name four pumps involved in Ca regulation. Explain each role

Answer 2

primary AT :
Plasma membrane Ca ATPase
Sacroplasmic reticulum ATPase

Secondary AT:
Na-Ca exchanger

Facilitated diffusion:
mt ca uniport

Question 3

Why could Na-K exchanger cause damage in ischemia?

Answer 3

na-k pump fails causing na influx 
depolarise cell 
NCX operation reverses
ca influx 
toxic

Question 4

Name 2 acid extruders

Answer 4

Na/H exchnager

na dependent cl/hco3 exchanger

Question 5

Name base extruders

Answer 5

cl/hco3 exchanger also known as band 3 the anionic exchanger

Question 6

Mechanisms of Cell volume regulation?

Answer 6

vary depending on cell general principle involved transport of osmotically active ions
mechanisms= electroneutral

Question 7

What pump do loop diuretics block in the proximal tube?

Answer 7

NKCC2 (na/k/cl in)

Question 8

What pump does thiazides block in distal convoluted tube?

Answer 8

NCCT (Na/Cl in)

Question 9

What pump does amiloride block in the distal convoluted tube and collecting duct?

Answer 9

ENaC

Question 10

What pumps does ADH act on in the collecting duct?

Answer 10

Aquaporin 2 increase water uptake
ROMK K out
ENac

Question 11

What pump is inhibited by spironolactone in collecting duct?

Answer 11

ROMK (k out)