Anitgen P&P- Pathways Flashcards
What are the following molecules important for?
Invariant chain
HLA-DM
HLA-DO
MARCH-1
MHC class 2 antigen processing and presentation
What fuses with the phagosome to form the MHC class 2 molecule?
Lysosome
What fuses with the lysosome/phagosome fused molecule to form the MHC class 2 molecule?
Endosome with CLIP
What catalyzes the release of CLIP and the binding of antigenic peptides in MHC 2 compartment (MIC)?
HLA-DM
HLA-DO acts as a NEGATIVE regulator of ___-__
HLA-DM
DR binds a peptide —> DM allows peptide exchange —> DO ________ DM from editing MHC 2 molecules
Prevents
What regulates MHC class 2 antigen processing and presentation?
MARCH-1
Which MHC class are the following molecules important for?
-Transporters associated with antigen processing (TAP)
-Endoplasm9c reticulum amino-peptidase associated with antigen processing (ERAAP)
-Proteosome
1
Which MHC class 1 molecule is responsible for transporting peptides generated from proteasome, that are from the cytosol, to the ER?
TAP
What do TAP 1 and 2 form?
Heterodimer
What is the expression of TAP enhanced by?
Interferon
The amino termini of the peptides produced by proteasome are too long and need to be trimmed by ______ before binding to MHC 1 molecules?
ERAAP
Whose expression is increased by IFN-y?
ERAAP
What has a 20S catalytic core and 2 19S regulatory caps?
Constitutive proteasome
What are the 2 outer ring subunits called located on the 20S catalytic core of the constitutive proteasome?
Alpha (7 of them)
Are the two outer rings- Alpha units- catalytic or not catalytic?
Not catalytic
What are the 2 inner ring subunits called located on the 20S catalytic core of the constitutive proteasome?
Beta (7 of them)
For Ubiquitin-proteasome system (UPS)-
What needs to be added with ubiquitin before processed by proteasome?
Proteins
What chain of UPS is recognized by the 19S cap?
Ubiquitin chain
What proteasome is induced by interferons and
PA28 proteasome-activator complex is induced by
IFN-y?
Immunoproteasome
What type of subunits are regulated accommodate antigen processing needs?
Proteasome
Exogenous antigens may enter the cytoplasm joining the ____________ antigen pathway, and vice versa
Endogenous
What 2 pathways interact extensively in the antigen processing pathways?
MHC 1 and 2
What type of antigens can be loaded on to MHC 1 molecules- CROSS PRESENTATION?
Exogenous
Only _________ DC subset can efficiently perform cross-presentation
Specific
What type of antigens are loaded on MHC 2 molecules- AUTOPHAGY PATHWAY
Endogenous
Which pathway has antigens that are processed into small peptides by the proteasome in the cytoplasm?
MHC 1
What pathways is: ingested proteins are broken up by lysosomal proteases into peptide fragment of varying lengths?
MHC 2
