8.4.2 Diagnosis of Heritable Conditions

Question 1

Define a DNA probe

Answer 1

Short, single strand of DNA, with bases complementary to a specific allele

Question 2

Describe how DNA probes can be used to view if an allele is present

Answer 2

• If probe is complementary to sample, DNA hybridisation occurs
• Wash sample to remove unbound probes
• Marker will be visualised if probe bound
• This means allele present

Question 3

What is attached often to DNA probe to diagnose hertiable conditions

Answer 3

Marker