2.1.3 Microscopy Flashcards
1
Q
Define magnification
A
How many times bigger an object appears
2
Q
Define resolution
A
The ability to distinguish two points next to each other
3
Q
If the resolution of a microscopic image is too low, what can be said about the light/electrons used
A
The wavelength is too long
4
Q
Magnification equation
A
Magnification = image size / actual size
5
Q
1cm = ?mm
A
10
6
Q
1mm = ?µm
A
1000
7
Q
1µm = ?nm
A
1000
8
Q
Measurement unit conversion image
A
9
Q
How to measure an object using a microscope and graticule
A
- Line up graticule with object
- Count how many epu for the measurement
- Calibrate graticule using stage micrometer - how many µm per epu
- Multiply the number of epu by the calibration factor
10
Q
Resolution of optical microscope
A
Low
11
Q
Resolution of SEM
A
Medium
12
Q
Resolution of TEM and ADVANTAGE
A
- High
- Allows organelles to be seen in greater detail
13
Q
Magnification of optical microscope
A
Low
14
Q
Magnification of TEM
A
High
15
Q
Magnification of SEM
A
Medium