2.1 Flashcards
eukaryotic cells
state 2 types of stains and what they can be used for
methylene blue - nuclei of animal cells
iodine - starch containing materials (plant cells)
define resolution
how close together two objects can be before they are seen as one
3 advantages of light microscopes
light and portable
cheap and assessable
can view living cells
3 disadvantages of electron microscope
specimen must be dead as they are viewed in a vacuum
expensive
preparing specimen is complicated
2 differences between TEM and SEM
TEM: 2D image
highest magnification
SEM: 3D image
lower magnification
how can you add colour to specimen in an electron microscope
add a heavy metal and the image will show the different density’s which are then coloured
state the 11 organelles inside a cell
centriole
SER
mitochondria
nuclear membrane
nucleolus
chromosomes
lysosomes
RER
vesical
golgi body
ribosomes
why is cholesterol needed in the cell membrane
for the fluidity of the membrane
contrast 70s and 80s ribosomes
80s: 40s + 60s subunits
eukaryotic cells
bigger
70s: 30s + 50s subunits
prokaryotic cells
smaller
what is the endosymbiotic theory
mitochondria and chloroplasts evolved from bacteria which got caught inside eukaryotic cells
what is the general structure and function of endoplasmic reticulum
interconnected network of folded membranes
formation + transport of proteins and lipids
why is it important for organelles to have separate membranes
to make it possible to have the right conditions for a particular reaction in one part of a cell and different conditions to suit other reactions elsewhere in the same cell
state 3 uses of the cell membrane
controlling what passes into & out of the cell
allowing fluids either side to have different compositions
where chemical processes take place
why do cell membranes need to be flexible
allow cell to change shape
water context
engulfing
break & fuse easily to allow vesicles through
what are vesicles
membrane ‘bags’ that hold secretions made in cells