Week 3 Bacterial Struc

Question 1

Hydrophobic substance acid fast

Answer 1

mycolic acid

Question 2

Halo like

Answer 2

Capsule

Question 3

Mechanism of Slime layer

Answer 3

Inhibits phagocytosis and host tissue

Question 4

Acetone alcohol

Answer 4

Decolorizer

Question 5

optimal growth of mesophiles

Answer 5

20° to 40° C

Question 6

is the most common solidifying agent or it is the solidifying agent in a medium

Answer 6

Agarose

Question 7

Made by adding a solidifying agent to the nutrients and water.

Answer 7

Solid (agar) medium

Question 8

Powder used for solidify agent

Answer 8

Agar powder

Question 9

1 genus of cell wall less bacteria

Answer 9

Mycoplasma

Question 10

1 structure unique to gram negative

Answer 10

Proteins, phospholipids and lipopolysaccharide

Question 11

Specific term for pair cocci

Answer 11

Diplococci

Question 12

Phase of growth curve that is constant

Answer 12

Stationary phase

Question 13

Exact ph range of media ideal for growth of most organism or Diagnostic laboratory media for bacteria are usually adjusted to a
final pH between

Answer 13

Neutral ph (7.0 -7.5 ph)

Question 14

Organism requires extra amount of carbon dioxide or organism that required extra amount of co2 is called?

Answer 14

Capnophilic organism

Question 15

Bacteria reproduced/replicate to which process

Answer 15

Binary fission

Question 16

Where Growth can be seen at aerobe or require oxygen for their growth.

Answer 16

Obligate aerobes

Question 17

2 components of peptidoglycan or 2 layers of peptidoglycan

Answer 17

N- acetyl- d- glucosamine and n-acetyl- d - muramic acid

Question 18

Carbohydrate present in agar

Answer 18

Sucrose and lactose

Question 19

Consistency based of blood agar

Answer 19

Solid media

Question 20

Type of water in culture media

Answer 20

Deionized water or distilled water

Question 21

Sterilizer process

Answer 21

Autoclave

Question 22

Most pathogenic bacteria grow best ph at a

Answer 22

Neutral ph

Question 23

Organism requires special requirement or an organism which special growth requirement are called?

Answer 23

Fastidious organism

Question 24

Type of medium used for motility

Answer 24

Semi-solid culture media

Question 25

Tissue sample should be ground using what process

Answer 25

Homogenization

Question 26

Endospore stain

Answer 26

Schaeffer fulton

Question 27

How many Swabs

Answer 27

2 swabs

Question 28

small, dormant (inactive), asexual spores that develop inside the bacterial cell (active vegetative cell) as a means of survival or do become vegetative when the harsh conditions are removed

Answer 28

Endospores

Question 29

differentiates vibro species

Answer 29

Sucrose in thiosulfate citrate bile salts sucrose agar

Question 30

this process deposit a mono layer of cellular element and bacteria on a slide

Answer 30

Cytocentrifugation

Question 31

this is use to decolorized in gram staining

Answer 31

Acetone alcohol

Question 32

Rod-shaped

Answer 32

Bacillus / bacilli

Question 33

the ph of the media can be tested using

Answer 33

Ph paper or ph meter

Question 34

highly refractile bodies in the cell

Answer 34

Spores

Question 35

enrichment media for the isolation of vibrio cholerae

Answer 35

Alkaline peptone water

Question 36

while obligate anaerobes is culture in a solid tube media growth can be seen

Answer 36

Bottom of tube

Question 37

10-20 degree celcius

Answer 37

Psychrophiles

Question 38

Primary stain of schaeffer fulton

Answer 38

Malachite green

Question 39

3 regions in gram negative -LPS

Answer 39

1. Antigenic - O specific polysaccharide
2. Core polysaccharide
3. Inner lipid A

Question 40

Medium for vibrio cholerae

Answer 40

Bile salt agar

Question 41

Nutrients are dissolved in water, and bacterial growth is indicated by a change in broth‟s appearance from clear to turbid

Answer 41

Liquid ( broth)