Week 3 Bacterial Struc Flashcards
Hydrophobic substance acid fast
mycolic acid
Halo like
Capsule
Mechanism of Slime layer
Inhibits phagocytosis and host tissue
Acetone alcohol
Decolorizer
optimal growth of mesophiles
20° to 40° C
is the most common solidifying agent or it is the solidifying agent in a medium
Agarose
Made by adding a solidifying agent to the nutrients and water.
Solid (agar) medium
Powder used for solidify agent
Agar powder
1 genus of cell wall less bacteria
Mycoplasma
1 structure unique to gram negative
Proteins, phospholipids and lipopolysaccharide
Specific term for pair cocci
Diplococci
Phase of growth curve that is constant
Stationary phase
Exact ph range of media ideal for growth of most organism or Diagnostic laboratory media for bacteria are usually adjusted to a
final pH between
Neutral ph (7.0 -7.5 ph)
Organism requires extra amount of carbon dioxide or organism that required extra amount of co2 is called?
Capnophilic organism
Bacteria reproduced/replicate to which process
Binary fission
Where Growth can be seen at aerobe or require oxygen for their growth.
Obligate aerobes
2 components of peptidoglycan or 2 layers of peptidoglycan
N- acetyl- d- glucosamine and n-acetyl- d - muramic acid
Carbohydrate present in agar
Sucrose and lactose
Consistency based of blood agar
Solid media
Type of water in culture media
Deionized water or distilled water
Sterilizer process
Autoclave
Most pathogenic bacteria grow best ph at a
Neutral ph
Organism requires special requirement or an organism which special growth requirement are called?
Fastidious organism
Type of medium used for motility
Semi-solid culture media
Tissue sample should be ground using what process
Homogenization
Endospore stain
Schaeffer fulton
How many Swabs
2 swabs
small, dormant (inactive), asexual spores that develop inside the bacterial cell (active vegetative cell) as a means of survival or do become vegetative when the harsh conditions are removed
Endospores
differentiates vibro species
Sucrose in thiosulfate citrate bile salts sucrose agar
this process deposit a mono layer of cellular element and bacteria on a slide
Cytocentrifugation
this is use to decolorized in gram staining
Acetone alcohol
Rod-shaped
Bacillus / bacilli
the ph of the media can be tested using
Ph paper or ph meter
highly refractile bodies in the cell
Spores
enrichment media for the isolation of vibrio cholerae
Alkaline peptone water
while obligate anaerobes is culture in a solid tube media growth can be seen
Bottom of tube
10-20 degree celcius
Psychrophiles
Primary stain of schaeffer fulton
Malachite green
3 regions in gram negative -LPS
- Antigenic - O specific polysaccharide
- Core polysaccharide
- Inner lipid A
Medium for vibrio cholerae
Bile salt agar
Nutrients are dissolved in water, and bacterial growth is indicated by a change in broth‟s appearance from clear to turbid
Liquid ( broth)
