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Culture Media Flashcards

1
Q

It is the media containing the containing the required nutrients for bacterial growth

A

Culture media

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2
Q

Use to Isolate and identification of micro-organisms

A

Culture media

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3
Q

Used to Perform anti-microbial sensitivity tests

A

Culture media

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4
Q

Hydrolyzed product of animal and plant proteins:

A

Peptone

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5
Q

It is common ingredients that Provides nitrogen; as well carbohydrates, nucleic acid fractions, minerals and vitamins.

A

Peptone

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6
Q

It is a common ingredient that Supply amino acids, vitamins and mineral salts.

A

Meat extract

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7
Q

Its is a common ingredient that is a bacterial growth stimulants

A

Yeast extract

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8
Q

It is a common ingredient that Phosphates as a source of phosphorus. And also Sodium chloride

A

Mineral salts

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9
Q

It is a common ingredient that Simple and complex sugars are a source of carbon and energy.

A

Carbohydrates

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10
Q

Sucrose in TCBS agar differentiates

A

Vibrio sp.

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11
Q

Lactose in MacConkey agar differentiates

A

Enterobacteria

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12
Q

It is a common ingredient that An inert polysaccharide of seaweed

A

Agar

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13
Q

It is a common ingredient Not metabolized by micro-organisms; solidifying agent

A

Agar

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14
Q

For High melting temperature of culture media is what degree Celsius

A

90-95 degree celsius

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15
Q

In what concentration is used to forms firm gel in agar

A

1.5% W/V concentration

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16
Q

In what concentration is used to forms semisolid gel

A

0.4-0.5% W/V concentration

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17
Q

What water must be used in the preparation of culture media.

A

Deionized water or distilled water

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18
Q

What is the 3 consistency of agar

A

Liquid (broth)
Solid (agar)
Semisolid

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19
Q

Nutrients are dissolved in water, and bacterial growth is indicated by a change in broth‟s appearance from clear to turbid

A

Liquid (broth)

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20
Q

Uses of liquid (broth)

A

Enrichment media
Biochemical testing media
Blood culture media

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21
Q

Made by adding a solidifying agent to the nutrients and water.

A

Solid (agar) media

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22
Q

is the most common solidifying agent.

A

Agarose

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23
Q

containing the agar is referred to as agar.

A

Petri dish

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24
Q

Stab/slope cultures in tubes and bottles

A

Solid agar media

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25
Description of Bacterial Colonies
Size Outline Elevation Transparency Surface Color Change
26
For special purposes where agar is added to media in concentrations that are too low to solidify them.
Semisolid culture media
27
Use in semisolid culture media
Enrichment media Motility media
28
Contain only basic nutrients required for the growth of ordinary organisms, and used as a general purpose media (base for preparation)
Basic /Simple / All Purpose Media
29
Media that contain some ingredients of unknown chemical composition are called complex media. (routine bacterial culture)
Complex Media
30
It is a complex media One common ingredient
Peptone
31
which are the water-soluble components of a substance, are also used in complex media
Extract
32
Commonly used complex medium, (in liquid form).
Nutrient broth
33
A simple basal liquid medium supports growth of many organisms. (general purpose media)
Nutrient broth
34
Types of Nutrient Broth:
Meat infusion broth Meat extract broth Digest broth.
35
Nutrient agar: Prepared by adding agar at a concentration of ________ to the nutrient broth. How many percentage of concentration
2% concentration
36
It is simplest and most common medium used routinely in microbiology laboratories
Nutrient broth
37
to grow nonfastidious bacteria nutrient agar is commonly referred to as
Agar medium
38
Prepared exclusively from pure chemical substances and their exact composition are known. (research purposes)
Synthetic or Chemically Defined Media
39
Prepared to meet the nutritional requirements of more bacteria by the addition of substances such as blood, serum or egg to a basal medium.
Enriched Media
40
Many bacteria are fastidious, requiring a medium that is even richer than nutrient agar commonly used in clinical laboratories. (whole blood)
Blood Agar
41
What is used for isolation in blood agar
streptococci, pneumococci, Haemophilus
42
Used to culture even more fastidious bacteria.
Chocolate agar
43
Used in isolation of chocolate agar
Neisseria (Meningococci and Gonococci) and Haemophilus.
44
Serum is added for enriching the medium.
Loeffler’s serum
45
Substance added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria
Enrichment Media
46
Is usually liquid and provides nutrients and environmental conditions that favor the growth of a particular microbe but not others.
Enrichment Media
47
Inhibits coliforms while allowing typhoid- paratyphoid bacilli to grow freely in fecal sample; Isolation of salmonella
Tetrathionate Broth
48
It is used for dysentery bacilli.
Selenite F (F for feces) Broth
49
It is used for Vibrio cholerae from feces.
Alkaline peptone water
50
Substance is added to a solid medium which inhibits the growth of unwanted bacteria but favors the growth of wanted bacteria
Selective Media
51
Used to isolate particular bacteria from specimens where mixed bacterial flora is expected.
Selective media
52
Acts as a selective agent for dysentery bacilli (isolation of Salmonella and Shigellae).
Desoxycholate citrate agar (DCA)
53
Used for Mycobacterium tuberculosis.
Lowenstein-Jensen medium
54
A selective agent allows the growth of only Vibrio cholerae and inhibits the growth of intestinal organisms.
Bile salt agar (BSA)
55
Contain an indicator which changes color when a bacterium grows in them.
Indicator Media
56
Incorporation of sulfite, S. typhi reduces sulfite to sulfide in the presence of glucose and the colonies of S. Typhi have a black metallic sheen.
Wilson and Blair medium
57
Indicates lactose fermenting property, lactose fermenter (LF) produces pink colonies and non-lactose fermenter (NLF) produces colorless colonies due to a neutral red indicator.
Macconkey agar
58
Has substances incorporated in it, enabling it to bring out differing characteristics of bacteria and thus helping to distinguish between them
Differential Media
59
is both differential and selective, contains peptone, meat extract, NACl, bile salt, lactose and neutral red indicator.
Macconkey agar
60
For the identification of most of the organisms, sugar fermentation reactions are carried out.
Sugar Media
61
are used to test fermentation.
Sugar media
62
is used „for characterization and identification of bacteria, particularly important in the study of Gram negative bacilli.
Carbohydrate fermentation
63
are routinely employed for fermentation tests.
Glucose, lactose, sucrose and mannitol
64
What is Color of the sugar media medium is
Yellow color
65
In sugar media the Acid production is indicated by the development of
Pink color
66
are used for organisms which are demanding in their growth requirements (fastidious organisms) like Streptococci, Pneumococci.
Hiss serum sugars
67
Media containing ingredients to prevent the overgrowth of commensals and ensure the survival of pathogenic bacteria when specimens cannot be cultured soon after collection.
Transport media
68
Used to grow anaerobic organisms, and contain reducing substances.
Anaerobic Media
69
sterilizes most agar and fluid culture media. 121 degree Celsius 15 psi for not less than 15 minutes
Autoclaving
70
sterilize media containing ingredients that would be inactivated at temperature over 100 C and re-melt previously bottled sterile agar media.
Steaming at 100C
71
sterilize media containing ingredients that would be inactivated at temperature over 100 C and re-melt previously bottled sterile agar media.
Steaming at 100C
72
sterilizes additives that are heat-sensitive and cannot be autoclaved.
Filtration

Bacte Quizes (16 decks)

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