Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacte Quizes > Culture Media > Flashcards

Culture Media Flashcards

1
Q

It is the media containing the containing the required nutrients for bacterial growth

A

Culture media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Use to Isolate and identification of micro-organisms

A

Culture media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Used to Perform anti-microbial sensitivity tests

A

Culture media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Hydrolyzed product of animal and plant proteins:

A

Peptone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

It is common ingredients that Provides nitrogen; as well carbohydrates, nucleic acid fractions, minerals and vitamins.

A

Peptone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

It is a common ingredient that Supply amino acids, vitamins and mineral salts.

A

Meat extract

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Its is a common ingredient that is a bacterial growth stimulants

A

Yeast extract

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

It is a common ingredient that Phosphates as a source of phosphorus. And also Sodium chloride

A

Mineral salts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

It is a common ingredient that Simple and complex sugars are a source of carbon and energy.

A

Carbohydrates

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Sucrose in TCBS agar differentiates

A

Vibrio sp.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Lactose in MacConkey agar differentiates

A

Enterobacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

It is a common ingredient that An inert polysaccharide of seaweed

A

Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

It is a common ingredient Not metabolized by micro-organisms; solidifying agent

A

Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

For High melting temperature of culture media is what degree Celsius

A

90-95 degree celsius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

In what concentration is used to forms firm gel in agar

A

1.5% W/V concentration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

In what concentration is used to forms semisolid gel

A

0.4-0.5% W/V concentration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What water must be used in the preparation of culture media.

A

Deionized water or distilled water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is the 3 consistency of agar

A

Liquid (broth)
Solid (agar)
Semisolid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Nutrients are dissolved in water, and bacterial growth is indicated by a change in broth‟s appearance from clear to turbid

A

Liquid (broth)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Uses of liquid (broth)

A

Enrichment media
Biochemical testing media
Blood culture media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Made by adding a solidifying agent to the nutrients and water.

A

Solid (agar) media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

is the most common solidifying agent.

A

Agarose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

containing the agar is referred to as agar.

A

Petri dish

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Stab/slope cultures in tubes and bottles

A

Solid agar media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Description of Bacterial Colonies

A

Size
Outline
Elevation
Transparency
Surface
Color
Change

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

For special purposes where agar is added to media in concentrations that are too low to solidify them.

A

Semisolid culture media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Use in semisolid culture media

A

Enrichment media
Motility media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Contain only basic nutrients required for the growth of ordinary organisms, and used as a general purpose media (base for preparation)

A

Basic /Simple / All Purpose Media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Media that contain some ingredients of unknown chemical composition are called complex media. (routine bacterial culture)

A

Complex Media

30
Q

It is a complex media One common ingredient

A

Peptone

31
Q

which are the water-soluble components of a substance, are also used in complex media

A

Extract

32
Q

Commonly used complex medium, (in liquid form).

A

Nutrient broth

33
Q

A simple basal liquid medium supports growth of many organisms. (general purpose media)

A

Nutrient broth

34
Q

Types of Nutrient Broth:

A

Meat infusion broth
Meat extract broth
Digest broth.

35
Q

Nutrient agar: Prepared by adding agar at a
concentration of ________ to the nutrient broth. How many percentage of concentration

A

2% concentration

36
Q

It is simplest and most common medium used routinely in microbiology laboratories

A

Nutrient broth

37
Q

to grow nonfastidious bacteria nutrient agar is commonly referred to as

A

Agar medium

38
Q

Prepared exclusively from pure chemical substances and their
exact composition are known. (research purposes)

A

Synthetic or Chemically Defined Media

39
Q

Prepared to meet the nutritional requirements of more bacteria by the addition of substances such as blood, serum or egg to a basal medium.

A

Enriched Media

40
Q

Many bacteria are fastidious, requiring a medium that is even richer than nutrient agar commonly used in clinical laboratories. (whole blood)

A

Blood Agar

41
Q

What is used for isolation in blood agar

A

streptococci, pneumococci, Haemophilus

42
Q

Used to culture even more fastidious bacteria.

A

Chocolate agar

43
Q

Used in isolation of chocolate agar

A

Neisseria (Meningococci and Gonococci) and Haemophilus.

44
Q

Serum is added for enriching the medium.

A

Loeffler’s serum

45
Q

Substance added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria

A

Enrichment Media

46
Q

Is usually liquid and provides nutrients and environmental conditions that favor the growth of a particular microbe but not others.

A

Enrichment Media

47
Q

Inhibits coliforms while allowing typhoid- paratyphoid bacilli to grow freely in fecal sample;
Isolation of salmonella

A

Tetrathionate Broth

48
Q

It is used for dysentery bacilli.

A

Selenite F (F for feces) Broth

49
Q

It is used for Vibrio cholerae from feces.

A

Alkaline peptone water

50
Q

Substance is added to a solid medium which inhibits the growth of unwanted bacteria but favors the growth of wanted bacteria

A

Selective Media

51
Q

Used to isolate particular bacteria from specimens where mixed bacterial flora is expected.

A

Selective media

52
Q

Acts as a selective agent for dysentery bacilli (isolation of Salmonella and Shigellae).

A

Desoxycholate citrate agar (DCA)

53
Q

Used for Mycobacterium tuberculosis.

A

Lowenstein-Jensen medium

54
Q

A selective agent allows the growth of only Vibrio cholerae and inhibits the growth of intestinal organisms.

A

Bile salt agar (BSA)

55
Q

Contain an indicator which changes color when a bacterium grows in them.

A

Indicator Media

56
Q

Incorporation of sulfite, S. typhi reduces sulfite to sulfide in the presence of glucose and the colonies of S. Typhi have a black metallic sheen.

A

Wilson and Blair medium

57
Q

Indicates lactose fermenting property, lactose fermenter (LF) produces pink colonies and non-lactose fermenter (NLF) produces colorless colonies due to a neutral red indicator.

A

Macconkey agar

58
Q

Has substances incorporated in it, enabling it to bring out differing characteristics of bacteria and thus helping to distinguish between them

A

Differential Media

59
Q

is both differential and selective, contains peptone, meat extract, NACl, bile salt, lactose and neutral red indicator.

A

Macconkey agar

60
Q

For the identification of most of the organisms, sugar fermentation reactions are carried out.

A

Sugar Media

61
Q

are used to test fermentation.

A

Sugar media

62
Q

is used „for characterization and identification of bacteria, particularly important in the study of Gram negative bacilli.

A

Carbohydrate fermentation

63
Q

are routinely employed for fermentation tests.

A

Glucose, lactose, sucrose and mannitol

64
Q

What is Color of the sugar media medium is

A

Yellow color

65
Q

In sugar media the Acid production is indicated by the development of

A

Pink color

66
Q

are used for organisms which are demanding in their growth requirements (fastidious organisms) like Streptococci, Pneumococci.

A

Hiss serum sugars

67
Q

Media containing ingredients to prevent the overgrowth of commensals and ensure the survival of pathogenic bacteria when specimens cannot be cultured soon after collection.

A

Transport media

68
Q

Used to grow anaerobic organisms, and contain reducing substances.

A

Anaerobic Media

69
Q

sterilizes most agar and fluid culture media. 121 degree Celsius 15 psi for not less than 15 minutes

A

Autoclaving

70
Q

sterilize media containing ingredients that would be inactivated at temperature over 100 C and re-melt previously bottled sterile agar media.

A

Steaming at 100C

71
Q

sterilize media containing ingredients that would be inactivated at temperature over 100 C and re-melt previously bottled sterile agar media.

A

Steaming at 100C

72
Q

sterilizes additives that are heat-sensitive and cannot be autoclaved.

A

Filtration

Bacte Quizes (16 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions