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Bacte Quizes > Lab Microscopy > Flashcards

Lab Microscopy Flashcards

(60 cards)

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1
Q

micron (μ) and millimicron (mμ) have been replaced by the terms

A

micrometer (μm) and nanometer (nm)

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2
Q

The size of a bacteria, fungi and unicellular parasites is usually expressed in?

A

Micrometer (um)

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3
Q

The size of a viruses is usually expressed in?

A

Nanometer (nm)

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4
Q

He was able to described the look and movement of microorganisms

A

Anton van leeuwenhoek

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5
Q

What kind of microscope has only one magnifying lens

A

Simple Microscopes

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6
Q

In simple microscope has Images appear about __to ___times larger than the object’s actual size

A

3 to 20 times

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7
Q

Leeuwenhoek’s simple microscopes had a maximum magnifying power of about _______

A

300x (300 times)

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8
Q

It contains more than one magnifying lens

A

Compound microscope

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9
Q

Who has given credit for being the first to conceptualize this magnify objects about 1,000 times

A

Hans Jansen and his son Zacharias

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10
Q

Photographs taken through the lens system of compound microscopes are called

A

Photomicrographs

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11
Q

Compound microscope is referred as a

A

Compound light microscope

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12
Q

Compound microscope has a wavelength of visible light (approximately _______ ) that limits the size of objects that can be seen

A

0.45 um

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13
Q

Location: At the top of the microscope
Function: the ocular lens has an x10 magnifying lens

A

Ocular lens

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14
Q

Location: Above the stage
Function: Holds the objective lenses

A

Revolving nosepiece

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15
Q

Location: Held in place above the stage by the revolving nosepiece
Function: Used to magnify objects placed on the stage

A

Objective lenses

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16
Q

Location: Directly beneath the nosepiece and objective lenses
Function: Flat surface on which the specimen is placed

A

Stage

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17
Q

Location: Beneath the stage
Function: Used to move the stage and microscope slide

A

Stage Adjustment knobs

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18
Q

Location: On the condenser
Function: Used to adjust the amount of light passing

A

Iris diaphragm

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19
Q

Location: Beneath the stage
Function: Contains a lens system that focuses light onto the specimen

A

Condenser

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20
Q

Location: Beneath the Condenser
Function: Controls the amount of light entering the condenser source

A

Collector lens with field diaphragm

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21
Q

Location: Front side of the Base
Function: Controls the amount of light emitted from the light

A

Rheostat control knob

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22
Q

Location: Attached to the field diaphragm
Function: Used to adjust the amount of light passing through the collector lens

A

Field diaphragm

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23
Q

Location: On the side of the base
Function: Turns the light source on and off

A

On/off Switch

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24
Q

Function: Contains the light source

A

Base

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25
Location: Beneath and behind the condenser Function: Used to adjust the height of the condenser
Condenser control knob
26
Location: On the arm of the microscope near the base Function: Used to focus the objective lenses
Fine and coarse adjustment knobs
27
Function: Supports the binocular body and the revolving nosepiece
Arm
28
Function: Holds the ocular lenses in their proper locations
Binocular body
29
objects are observed against a bright background. Used in clinical laboratory
Brightfield Microscopes
30
condenser is replaced with what is known as a darkfield condenser, illuminated objects are seen against a dark background
Darkfield Microscopes
31
Scanner has magnification of
4x
32
Low power objective has magnification of
10x
33
High power objective has magnification of
40x
34
Oil immersion objective has magnification of
100x
35
The total magnification of scanner is
40x
36
The total magnification of low power objective is
100x
37
The total magnification of high power objective is
400x
38
The total magnification of oil immersion objective is
1000x
39
The magnification of eyepiece or ocular lens
10x or 12.5x
40
Diagnostic of syphilis uses serologic test or microscopy
Darkfield microscope
41
used to observe unstained living microorganisms
Phase-contrast Microscopes
42
used to observed cellular processes
Phase-contrast Microscopes
43
contain a built-in Ultraviolet (UV) Light Source
Fluorescence Microscopes
44
When UV light strikes certain dyes and pigments, these substances emit a longer wavelength light, causing them to glow against a dark background
Fluorescence Microscopes
45
often used in immunology laboratories to demonstrate that antibodies stained with a fluorescent dye have combined with specific antigen
Fluorescence microscope
46
In Fluorescence Microscopes what dye is used to stained
Fluorescent dye
47
capable to view viruses
Electron microscope
48
use an electron beam as a source of illumination and magnets to focus the beam
Electron microscopes
49
have a much greater resolving power than compound light microscopes (wavelength of electrons traveling in a vacuum is much shorter than the wavelength of visible light)
Electron microscope
50
Two types of electron microscopes:
Transmission electron microscopes and scanning electron microscopes.
51
Used to view the inside of your cell; inferior of the cell
Transmission Electron Microscopes
52
Specimen must be very thin and the electron beam will pass through the specimen
Transmission electron microscope
53
Transmission electron microscope has object can be magnified up to approximately
1 million times
54
specimen is placed at the bottom of the column
Scanning Electron Microscopes
55
Electrons that bounce off the surface of the specimen are captured by detectors, and an image of the specimen appears on a monitor
Scanning electron microscopes
56
used to observe the outer surfaces of specimens
Scanning electron microscopes
57
most powerful microscope
Atomic force microscope
58
observe living cells at extremely high magnification and resolution under physiological conditions
Atomic force microscope
59
possible to observe single live cells in aqueous solutions where dynamic physiological processes can be observed in real time
Atomic force microscope
60
true three-dimensional surface profile
Atomic Force Microscopes

Bacte Quizes (16 decks)

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