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Bacte Quizes > Lab Microscopy > Flashcards

Lab Microscopy Flashcards

1
Q

micron (μ) and millimicron (mμ) have been replaced by the terms

A

micrometer (μm) and nanometer (nm)

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2
Q

The size of a bacteria, fungi and unicellular parasites is usually expressed in?

A

Micrometer (um)

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3
Q

The size of a viruses is usually expressed in?

A

Nanometer (nm)

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4
Q

He was able to described the look and movement of microorganisms

A

Anton van leeuwenhoek

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5
Q

What kind of microscope has only one magnifying lens

A

Simple Microscopes

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6
Q

In simple microscope has Images appear about __to ___times larger than the object’s actual size

A

3 to 20 times

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7
Q

Leeuwenhoek’s simple microscopes had a maximum magnifying power of about _______

A

300x (300 times)

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8
Q

It contains more than one magnifying lens

A

Compound microscope

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9
Q

Who has given credit for being the first to conceptualize this magnify objects about 1,000 times

A

Hans Jansen and his son Zacharias

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10
Q

Photographs taken through the lens system of compound microscopes are called

A

Photomicrographs

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11
Q

Compound microscope is referred as a

A

Compound light microscope

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12
Q

Compound microscope has a wavelength of visible light (approximately _______ ) that limits the size of objects that can be seen

A

0.45 um

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13
Q

Location: At the top of the microscope
Function: the ocular lens has an x10 magnifying lens

A

Ocular lens

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14
Q

Location: Above the stage
Function: Holds the objective lenses

A

Revolving nosepiece

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15
Q

Location: Held in place above the stage by the revolving nosepiece
Function: Used to magnify objects placed on the stage

A

Objective lenses

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16
Q

Location: Directly beneath the nosepiece and objective lenses
Function: Flat surface on which the specimen is placed

A

Stage

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17
Q

Location: Beneath the stage
Function: Used to move the stage and microscope slide

A

Stage Adjustment knobs

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18
Q

Location: On the condenser
Function: Used to adjust the amount of light passing

A

Iris diaphragm

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19
Q

Location: Beneath the stage
Function: Contains a lens system that focuses light onto the specimen

A

Condenser

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20
Q

Location: Beneath the Condenser
Function: Controls the amount of light entering the condenser source

A

Collector lens with field diaphragm

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21
Q

Location: Front side of the Base
Function: Controls the amount of light emitted from the light

A

Rheostat control knob

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22
Q

Location: Attached to the field diaphragm
Function: Used to adjust the amount of light passing through the collector lens

A

Field diaphragm

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23
Q

Location: On the side of the base
Function: Turns the light source on and off

A

On/off Switch

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24
Q

Function: Contains the light source

A

Base

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25
Q

Location: Beneath and behind the condenser
Function: Used to adjust the height of the condenser

A

Condenser control knob

26
Q

Location: On the arm of the microscope near the base
Function: Used to focus the objective lenses

A

Fine and coarse adjustment knobs

27
Q

Function: Supports the binocular body and the revolving nosepiece

A

Arm

28
Q

Function: Holds the ocular lenses in their proper locations

A

Binocular body

29
Q

objects are observed against a bright background. Used in clinical laboratory

A

Brightfield Microscopes

30
Q

condenser is replaced with what is known as a darkfield condenser, illuminated objects are seen against a dark background

A

Darkfield Microscopes

31
Q

Scanner has magnification of

A

4x

32
Q

Low power objective has magnification of

A

10x

33
Q

High power objective has magnification of

A

40x

34
Q

Oil immersion objective has magnification of

A

100x

35
Q

The total magnification of scanner is

A

40x

36
Q

The total magnification of low power objective is

A

100x

37
Q

The total magnification of high power objective is

A

400x

38
Q

The total magnification of oil immersion objective is

A

1000x

39
Q

The magnification of eyepiece or ocular lens

A

10x or 12.5x

40
Q

Diagnostic of syphilis uses serologic test or microscopy

A

Darkfield microscope

41
Q

used to observe unstained living microorganisms

A

Phase-contrast Microscopes

42
Q

used to observed cellular processes

A

Phase-contrast Microscopes

43
Q

contain a built-in Ultraviolet (UV) Light Source

A

Fluorescence Microscopes

44
Q

When UV light strikes certain dyes and pigments, these substances emit a longer wavelength light, causing them to glow against a dark background

A

Fluorescence Microscopes

45
Q

often used in immunology laboratories to demonstrate that antibodies stained with a fluorescent dye have combined with specific antigen

A

Fluorescence microscope

46
Q

In Fluorescence Microscopes what dye is used to stained

A

Fluorescent dye

47
Q

capable to view viruses

A

Electron microscope

48
Q

use an electron beam as a source of illumination and magnets to focus the beam

A

Electron microscopes

49
Q

have a much greater resolving power than compound light microscopes (wavelength of electrons traveling in a vacuum is much shorter than the wavelength of visible light)

A

Electron microscope

50
Q

Two types of electron microscopes:

A

Transmission electron microscopes and scanning electron microscopes.

51
Q

Used to view the inside of your cell; inferior of the cell

A

Transmission Electron Microscopes

52
Q

Specimen must be very thin and the electron beam will pass through the specimen

A

Transmission electron microscope

53
Q

Transmission electron microscope has object can be magnified up to approximately

A

1 million times

54
Q

specimen is placed at the bottom of the column

A

Scanning Electron Microscopes

55
Q

Electrons that bounce off the surface of the specimen are captured by detectors, and an image of the specimen appears on a monitor

A

Scanning electron microscopes

56
Q

used to observe the outer surfaces of specimens

A

Scanning electron microscopes

57
Q

most powerful microscope

A

Atomic force microscope

58
Q

observe living cells at extremely high magnification and resolution under physiological conditions

A

Atomic force microscope

59
Q

possible to observe single live cells in aqueous solutions where dynamic physiological processes can be observed in real time

A

Atomic force microscope

60
Q

true three-dimensional surface profile

A

Atomic Force Microscopes

Bacte Quizes (16 decks)

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