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Lab Smear And Stain Flashcards

1
Q

A Danish bacteriologist who developed a staining technique that separates bacteria into two groups

A

Hans Christian gram

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2
Q

Two group that Christian Gram developed

A

Gram positive and gram negative

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3
Q

The procedure is based on the ability of microorganisms to retain the purple color of crystal violet during decolorization with alcohol.

A

Gram stain

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4
Q

are decolorized by the alcohol, losing the purple color of crystal violet.

A

Gram negative bacteria

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5
Q

are not decolorized and remain purple

A

Gram positive bacteria

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6
Q

What are the reagents of gram stain

A

Crystal violet, iodine, acetone alcohol and safranin

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7
Q

Gram positive cocci is color

A

Violet or purple

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8
Q

Gram negative bacilli is color

A

Pink

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9
Q

In gram positive crystal violet it stained as

A

Violet or purple

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10
Q

In gram positive in iodine it stained as

A

Violet or purple

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11
Q

In gram positive acetone alcohol it stained as

A

Violet or purple

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12
Q

In gram positive safranin it stained as

A

Violet or purple

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13
Q

What is the primary stain/initial stain of gram stain

A

Crystal violet

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14
Q

What is the mordant of gram stain

A

Gram’s iodine

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15
Q

What is the decolorizer of gram stain

A

Acetone alcohol

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16
Q

What is the counterstain/secondary stain of gram stain

A

Safranin red

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17
Q

In gram negative crystal violet stained as

A

Violet or purple

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18
Q

In gram negative iodine it stained as

A

Violet or purple

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19
Q

In gram negative acetone alcohol stained as

A

Colorless

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20
Q

In gram negative safranin stained as

A

Pink

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21
Q

duration time of crystal violet in gram staining

A

1 mins

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22
Q

duration time of iodine in gram staining

A

2 mins

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23
Q

Duration time of decolorizer in gram staining

A

30 sec

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24
Q

Duration time of safranin red in gram staining is

A

1 min

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25
Q

Commonly dyes used in bacteriology

A

Basic Dyes

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26
Q

Cationic (pentavalent nitrogen) that bind to negatively charged molecules like nucleic acids and protein

A

Basic dyes

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27
Q

Anionic or possess negatively charged groups (carboxyls and phenolic) that bind to positively charged cell structures.

A

Acid Dyes

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28
Q

Stains cytoplasm (histopath)

A

Acid dyes

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29
Q

A single stain is used.

A

Simple staining

30
Q

It is directed towards coloring the forms and shapes present.

A

Simple staining

31
Q

It divides bacteria into separate groups.

A

Differential Staining

32
Q

Smear procedure from plated colony

A
  1. Label your slide
  2. Place a loopful of normal saline solution
  3. Emulsify on isolated colony. air dry
  4. Heat fix
33
Q

Smear procedure from broth

A
  1. Label your slide
  2. Place a loopful of broth. Air dry
  3. Heat fix
34
Q

Smear procedure from urine

A
  1. Label your slide
  2. Place a loopful of mixed well urine. Air dry
  3. Heat fix
35
Q

Smear procedure from throat swab

A
  1. Label your slide
  2. Collect specimen using sterile cotton swab
  3. Roll the swab gently back and forth
  4. Heat fix
36
Q

Simple staining procedure

A
  1. Flood the smear with methylene blue
  2. Set timer for 1 min
  3. Remove excess stain using gentle stream of water
  4. Blot dry and view under microscope
37
Q

Give the 5 streaking methods

A
  1. Interrupted streak
  2. Overlap streak
  3. Multiple interrupted streak
  4. Multiple inoculation
  5. Radial streak
38
Q

What kind of streaking no need aseptic procedure in between

A

Interrupted streak and multiple interrupted streak

39
Q

How many quadrants are there in interrupted streak

A

2 quadrants

40
Q

What kind of streak needs aseptic procedure after 1 streak

A

Overlap streak

41
Q

How many quadrants are there in overlap streak

A

3 quadrants

42
Q

In semi-quantitative reporting what growth on 1 quadrant means

A

Light growth

43
Q

In semi-quantitative what growth on 1 and 2 quadrants means

A

Moderate growth

44
Q

In semi-quantitative what growth on 1, 2 &3 quadrants means

A

Heavy growth

45
Q

What kind of streak do need to apply aseptic technique in every quadrant

A

Multiple inoculation

46
Q

What kind of streak need loopful of broth for streaking

A

Radial streak

47
Q

In heat fixing how many times do we need to do

A

3 to 4 times

48
Q

What 3 kinds of tube do we use to isolate colonies

A

Butt/slant, slant and butt

49
Q

Butt is used for

A

Motility testing and enrichment

50
Q

Butt/slant and slant is used for

A

Isolation of colonies

51
Q

What motion do we use for isolating in plates

A

Serpentine motion

52
Q

In butt tube how do we isolate them

A

Stab motion

53
Q

In radial streak degrees needed in between streak

A

10 degree angle

54
Q

In interrupted streak after doing the 1st streak how many degree angle will you turn before the 2nd streak

A

180 degree angle

55
Q

In overlap streak after doing the 1st streak how many degree angle will you turn before doing the 2 and 3 qudrants

A

90 degree angle

56
Q

What hands do we use to hold the cap of the tube

A

Pink finger of right hand

57
Q

Used for transferring from source to plated or slanted media.

A

Inoculating loop

58
Q

Use to spread the specimen by streaking

A

Inoculating loop

59
Q

Intended to transfer specimen to plated or slanted (butt and slanted)

A

Inoculating needle

60
Q

Used in Specific measure and volume

A

Calibrated loop

61
Q

0.001 or 0.01 ml for urine specimen to know if significant or not significant

A

Calibrated loop

62
Q

For fungal culture

63
Q

BHI is for liquid or broth

A

Brain heart infusion

64
Q

MRVP for liquid or broth

A

Methyl red vouges-proskauer

65
Q

SIM for butt

A

Sulfide indole motility

66
Q

SCA for slant

A

Simmon citrate agar

67
Q

TSI for butt/slant

A

Triple sugar iron

68
Q

LIA for butt/slant

A

Lysine iron agar

69
Q

In gram stain what is the size of the stain

A

Thumb size

70
Q

In smear procedure what is the size of the stain

A

25 cents Coin

71
Q

What substance are being decolorized in afb staining

A

Mycolic acid

Bacte Quizes (16 decks)

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