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Bacte Quizes > Autoclaving And Sterilization > Flashcards

Autoclaving And Sterilization Flashcards

1
Q

What is the principle of Autoclaving ?

A

heat under steam pressure

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2
Q

It is at this temperature in which all microorganisms (except for prions) and their endospores are destroyed within approximately 15 minutes

A

Autoclaving

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3
Q

In autoclaving what is recommended for media, liquids, utensils, glass pipettes (degree, psi, and mins)

A

121 °C, 15 psi for 15 minutes

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4
Q

In autoclaving what is recommended for decontaminating medical wastes (degree, psi, and mins)

A

132 °C, 15 psi for 30-60 minutes

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5
Q

the main component of a steam autoclave, it is where the materials to be sterilized are placed

A

Pressure chamber

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6
Q

the purpose of the this is to seal the chamber and maintain the sterilizing conditions within the autoclave.

A

Lid

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7
Q

it indicates the pressure inside the autoclave

A

Pressure Gauge

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8
Q

pressure within the autoclave can be controlled by lifting the whistle and releasing certain amount of vapor

A

Pressure releasing unit/Whistle

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9
Q

it is present underneath the chamber to heat the water and generate steam

A

Steam generator

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10
Q

Sterilizethematerialsinside the chamber using moist heat and pressure

A

Autoclave

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11
Q

The high pressure ensures the rapid penetration of heat into deeper parts of the material

A

Autoclave

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12
Q

The moisture causes denaturation of microbial proteins

A

Autoclave

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13
Q

Once sterilization is completed, pressure inside the chamber is released through the whistle

A

Autoclave

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14
Q

9 Steps in running an Autoclave

A
  1. Check the inside of the autoclave if there are items left from the previous cycle
  2. Place sufficient amount of water inside the chamber
  3. The materials to be sterilized are then placed inside
  4. The lid is closed using screws to ensure airtight condition, and the electric heater is switched on
  5. The water then boils and the pressure is allowed to reach the designated level
  6. Once the pressure is reached, the whistly blows to remove excess pressure from the chamber
  7. After the whistle, the autoclave is run for a holding period (15 minutes or more)
  8. The heater is switched off and the autoclave is allowed to cool until the pressure gauge indicates the pressure inside has lowered down
  9. The lid is opened and the sterilized materials are taken out of the chamber
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15
Q

Used in order to sterilize materials in the laboratory such as culture media, glassware and other instruments

A

Autoclave

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16
Q

Used in order to sterilize medical wastes that may contain dangerous microbes

A

Autoclave

17
Q

Precautions in using the Autoclave

A

The autoclave should not be overcrowded in order to ensure that all parts of the material can be penetrated by the steam
• Wastes and clean items should be autoclaved separately
• Do not attempt to open the lid when the autoclave is running
• Avoid placing materials that might melt inside the autoclave (plastic trays, etc.)

18
Q

involves the direct application of high heat in materials such as inoculating loops. This can be accomplished using an open flame (a) or an incinerator (b)

A

Direct flaming

19
Q

How many celsius is oover heating

A

160 to170 degrees Celsius for 1.5 to 2 hours Aseptic

20
Q

creates free radicals that causes intracellular damage

A

Radiation

21
Q

causes mutation in the DNA; destroys vegetative cells and endospores

A

Ionizing radiation

22
Q

Used to “pasteurize” meat products

A

Ionizing radiation

23
Q

Utilizes gamma rays and x-rays

A

Ionizing radiation

24
Q

damages cellular DNA

A

Non-ionizing radiation

25
Q

Used on exposed surfaces and rooms

A

Non-ionizing radiation

26
Q

Utilizes ultraviolet rays

A

Non-ionizing radiation

27
Q

first widely used antiseptic and disinfectant; destroys plasma membranes and denature cell proteins

A

Phenol

28
Q

denatures proteins and causes dissolution of lipid membranes; effective at 60-90% concentration

A

Alcohol

29
Q

used at 1:10 dilution; 3 minutes contact time

A

Chlorine (hypochlorite)

Bacte Quizes (16 decks)

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