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Vibrio Flashcards

1
Q

species has been associated with large epidemics and bandencs

A

Vibrio

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2
Q

infections may play a role in Guillain-Barre (gbs) which occurs when the immune system harms the body’s nerves, leading to paralysis

A

Campylobacter

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3
Q

can cause ulcers and is linked to
gastric carcinoma

A

Helicobacter pylori

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4
Q

is commonly found in a wide variety of aquatic
environments, including fresh water, brackish or estuarine water, and marine or salt water

A

Vibrio

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5
Q

Most commonly isolated species in vibrionaceae

A
  1. Vibrio cholerea
  2. Vibrio parahaemolyticus
  3. Vibrio vulnificus
  4. Vibrio alginolyticus
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6
Q

Vibrio: MICROSCOPIC MORPHOLOGY

A

Curved (comma)
Asporogenous (nonspore)
In broth - possess polar, sheathed flagella
In solid media - peritrichous, unsheathed flagella

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7
Q

Vibrio clinical manifestation

A
  1. consumption of raw seafood
  2. immigration or foreign travel
  3. Gastroenteritis with cholera-ke or rice-water
    Stool
  4. AccIdental trauma incurred during contact associated water
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8
Q

Does not grow on MAC and glucose fermenter

A

Vibrio

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9
Q

Routine media used in vibrio

A

Thiosulfate-citrate-bile salts-sucrose agar (TCBS)

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10
Q

Vibrio general characteristics

A
  • Observed as a mucoid “stringing”
  • halophilic or “salt-loving” (except v. Cholorea and v. Mimicus)
  • Require the addition of sodium
  • Glucose fermenter
  • Does not grow mac
  • facultative anaerobe
  • catalase negative (v. Metschnikovii)
  • oxidase positive (v. Metschnikovii)
  • reduce nitrate to nitrite
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11
Q

Serotype of india

A

Ogawa

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12
Q

Serotype of Philippine

A

Inaba

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13
Q

Serotype of japan

A

Hikojima

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14
Q

causative agent of cholera

A

Vibrio cholerea O1 (asiatic cholera or epidemic cholera

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15
Q

is a disease of major public health significance for centuries.

A

Cholera

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16
Q

in what place do cholera prevalent in the

A

Bengal region of india and bangladesh

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17
Q

Spread through contaminated water

A

Cholera

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18
Q

The disease manifests in acute cases as a severe gastroenteritis accompanied by vomiting and followed by diarrhea

A

Cholera

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19
Q

Rice watery stool contain

A

Mucus flecks

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20
Q

cholera responsible for

A

cholera toxin or choleragen (powerful enterotoxin)

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21
Q

Treatment and management for cholera

A

Intravenous and oral fluid

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22
Q

Cholera Can be shorten by antibiotics such as

A

Azithromycin and ciprofloxacin

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23
Q

Two types of Cholera:

A
  1. Classical type of cholera
  2. El tor type of cholera
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24
Q

Commonlv known as Summer Diarrhea in Japan

A

Vibrio parahaemolyticus

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25
Q

Second most common Vibrio species implicated in gastroenteritis

A

Vibrio parahaemolyticus

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26
Q

What type of vibrio is self-limited disease

A

Vibrio parahaemolyticus

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27
Q

Vibrio parahaemolyticus symptoms begin hours after ingestion of contaminated seafooc

A

24-48 hours

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28
Q

Produces a heat-stable hemolysin that is able to lyse human erythrocytes in a special, high salt mannitol medium

A

Kanagawa-toxin positive

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29
Q

What meduim is used in kanagawa-toxin positive

A

Wagatsuma agar

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30
Q

Least pathogenic; most frequently isolated

A

Vibrio alginolyticus

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31
Q

Two categories of infection: primary septicemia and wound infections

A

Vibrio vulnificus

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32
Q

Transport media for vibrio spp.

A

Carry-blair media

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33
Q

Recommended selective media for vibrio

A

Thiosulfate Citrate
Bile Salt Sucrose Agar (TCBS)

34
Q

Difterentiates sucrose-termenting yellow colonies) species from nonsucrose-termenting (green colonies)
species

A

Thiosulfate citrate bile salt-sucrose agar (TCBS)

35
Q

Enrichment procedure for vibrio

A

alkaline peptone water with 1% sodium chloride can be inoculated at least 20 ml and incubated for 5 to 8 hours at 35° C

36
Q

What non-lf colonies in vibrio

A

Macconkey agar

37
Q

What hemolysis Is the SBA USED IN VIBRIO

A

A and B hemolysis

38
Q

agar can give false-positive oxidase reactions in vibrio

A

Mac and cefsulodin irgasan novobiocin (cin)

39
Q

colonies - medium to large colonies that appear smoon, opaque, and Inaescent witn a greenisn hue in vibrio

A

Sba and cap

40
Q

Non-sucrose
Sucro serermenter
Fermenter

A

V. mimIcus,
V. parahaemolyticus,
V. damsela
and most V. vulnificus strain
Some V. Vulnificus strain

41
Q

Sucrose fermenter

A
  • V cholerae
  • V. alginolyticus,
  • V. fluvialis.
  • V. Furnissii,
    V. cincinnatiensis
  • V. metschnikovii
42
Q

Prefers aquatic habitats; usually freshwater, estuarine and marine environments

43
Q

Aeromonas major clinical species

A

A. hydrophila
A. Sobria
A. caviae - most commonly isolated specie

44
Q

Frequently isolated from animal meat products

45
Q

Aeromonas: GENERAL CHARACTERISTICS

A

Ubiquitous
Oxidase + ) positive
Glucose fermenter
Gram-neaative rods
Motile with single tuft of flagella (Lopotrichous’ widelv distributed in tresnwater. estuarine, and marine environments worldwide

46
Q

Aeromonas: CLINICAL MANIFESTATION

A

Enteric pathogens (different from Salmonella. Shiaella)
cause diarrheal disease
Diarrhea diarrheal infection is usually self-limiting
Extraintestinal infections - septicemia and wound infections
Most common presentation of wound infection
cellulitis

47
Q

most frequently associates with
gastrointestinal infections

48
Q

was formerly classified with the Vibrios
because of its Oxidase +) and microscopic
moronooav.

A

Campylobacter

49
Q

It colonizes the stomacn for a long time, causing a low-grade inflammatory process

A

Peptic ulcer (chronic superficial gastritis)

50
Q

Most clinically relevant species: campylobacter

A

C. jejuni subsp. jejuni
C. Coli
C. Fetus

51
Q

Mode of transmission of campylobacter

A

Direct contact
Person to person

52
Q

an autoimmune disorder
characterized by acute paralysis caused by damage to
the beronerd nervous systen

A

Guillain barre syndrome (GBs)

53
Q

most common cause of bacterial gastroenteritis
worldwide

54
Q

the causative agent of bacteremia

55
Q

major cause of type B gastritis or peptic ulcer

A

Helicobacter pylori

56
Q

Important species of helicobacter

A

Helicobacter pylori

57
Q

Delay in processing of stool specimen transport
Meduim

A

Carry-blair media

58
Q

Transport medium for Helicobacter pylori

A

Stuart medium

59
Q

Specimen for Campylobacter causing gastroenteritis
(C. jejuni:

A

Stool
Rectal swab (less preferred)

60
Q

Specimen of choice of C. fetus

A

Blood with incubation 35 - 37 degree

61
Q

Specimen for Helicobacter

A

Gastric biopsy material

62
Q

Commonly used selective media for C. jejuni:

63
Q

Other media used: campylobacter

A

Butzler medium
Skirrow medium

64
Q

commonly usea media тог н. рут0r

A

CAP
Brucella agar with 5% horse red blood cells (non selective)

65
Q

Selective meduim for helicobacter

A

Skirrow’s agar

66
Q

Incubation temperature: Campylobacter jejuni

67
Q

Incubation emperature:
Helicobacter pylori

68
Q

Requires a noncapnophilic and capnophilic
environment campylobacter

A

5 % O2
10% CO2

69
Q

Requires a noncapnophilic and capnophilic
environment helicobacter

A

5-10% O2
5-12% CO2

70
Q

May appear as long spirals or ‘S’ or seagull-wing shapes

A

Campylobacter SPP.

71
Q

Stain poorly on gram-stained smears

A

Campylobacter spp.

72
Q

recommended as a counter stain of campylobacter

A

Carbol fuchsin

73
Q

“Darting” motility (corkscrew movement)

A

Campylobacter

74
Q

Curved or U-shaned

A

Helicobacter spp.

75
Q

Motile with multiple flagella at one pole

A

Helicobacter

76
Q

What species Is morphology smoot, convex, translucent colonies

77
Q

What species is morphology moist, runny looking, and spreading and Some are round and raised and others may be flat also non hemolytic

A

C. Jenuni and other enteric Campylobacter

78
Q

2 oxidase positive

A

H. Pylori and C. Jejuni

79
Q

What test is used to Differentiate h. Pylori
And c. Jejuni

A

Urease test

80
Q

Urease test positive and negative

A

Positive - h. Pylori
Negative - c. Jejuni

81
Q

Hippurate hydrolysis test positive

Bacte Quizes (16 decks)

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