Virus Purification and Concentration

Question 1

What piece of equipment has played a vital role in virology, that efficiently sediments even the smallest viruses?

Answer 1

Ultracentrifuge

Question 2

What are ultracentrifuges used for?

Answer 2

Concentration and Purification of viruses

Question 3

Which type of centrifugation is used for separation of virus particles based on their mass?

Answer 3

Rate- Zonal Centrifugation

Question 4

Via Rate- Zonal Centrifugation, virus particles move at different rates depending on their _______.

Answer 4

Mass

Question 5

Particles are separated solely on the basis of their __________ _________, in Isopycnic Centrifugation.

Answer 5

Buoyant density

Question 6

Which centrifugation method separates particles based on buoyant density?

Answer 6

Isopycnic Centrifugation. (also called buoyant or equilibrium separation)

Question 7

What are the 4 ways viruses can be purified?

Answer 7

1) Ultracentrifuge
2) Rate- Zonal Centrifugation
3) Isopycnic Centrifugation
4) Membrane Chromatography