Cultivation of Viruses Flashcards
T/F: Viruses have the genetic capability to multiply by division.
FALSE!
–They DO NOT have the genetic capability to multiply by division.
Outside the host cell, viruses are ________/_________ particles.
inert/ dormant
What do viruses need in order to replicated and generate the next progeny of viruses?
A living host cell
What does the virus do once inside the host cell?
the virus hijacks and utilizes the host cell machinery to produce its proteins and nucleic acid for the next generation of virus .
What are the possible ways to Grow/ Cultivate viruses?
1) Cell/ Tissue Culture
2) Inoculated in Embryonate Egg
3) Laboratory Animals
Monolayer Cultures
When the bottom of the CULTURE vessel is COVERED WITH a CONTINUOUS LAYER OF CELLS, usually one cell in thickness.
Primary Cell Culture
This is the maintenance of growth of cells dissociated DIRECTLY FROM the PARENTAL TISSUE (such as kidney or liver) of human or animal origin.
What is the best culture system for isolation and propagation of viruses?
Primary Cell Culture
Which cultivation method, of viruses, is used in producing viral vaccines?
Primary Cell Culture
Secondary Culture/ Transfer Culture cell line includes:
1) Finite/ Dipoid Cell lines (Can be used for vaccine production)
2) Continuous Cell lines ( Can NOT be used for vaccine production because it is directly derived from cancer cells)
Subculture (or passage)
refers to the transfer of cells from one culture vessels to another culture vessel (it is apart of Secondary Culture/ Transfer Culture)
What is periodically required to provide fresh nutrients and growing space for continuously growing cell lines?
Secondary/ Transfer Culture
Finite/ Diploid Cell Lines
Cell lines which have a LIMITED LIFE SPAN and go through a LIMITED NUMBER of CELL GENERATIONS.
- Derived mainly from embryos or from secondary cell cultures
- CAN BE USED FOR VACCINE PRODUCTION
Continuous Cell Line
Acquire the ability to divide indefinitely (also knows as Immortal or Heteroploid cell line).
- Derived directly FROM CANCER CELLS!
- Can NOT be used for vaccine production
Which cell line does the FDA prohibit the use of in vaccine production?
Continous/ Immortal/ Heteroploid Cell Line
