Unit Four - Cells - Microscope

Question 1

Base

Answer 1

Supports the Microscope

Question 2

Stage

Answer 2

Table where the slide is placed

Question 3

Light Source

Answer 3

Illuminates Specimen for Clear Viewing

Question 3

Diaphragm (2)
(What it looks like, function)

Answer 3

1. Wheel shaped device
2. Regulates amount of light passing through the specimen

Question 4

Revolving Nosepiece (2)

Answer 4

1. Revolving section
2. Carries the objective lenses

Question 5

Objective Lenses (2)

Answer 5

1. Different Magnification Powers
2. To form an enlarged image

Question 5

Stage Clips

Answer 5

Secure slide on stage

Question 6

Ocular (2)

Answer 6

1. Brings light rays into focus
2. Magnifies image formed from Objectives

Question 7

Coarse Adjustment Knob (2)

Answer 7

1. Moves Stage Up and Down
2. Large Focusing

Question 8

Fine Adjustment Knob (2)

Answer 8

1. Moves Stage Slightly
2. For Precise Focusing

Question 9

How do you carry the microscope (3)

Answer 9

1. Grasp arm and under the base
2. Always hold microscope in an upright position so the eyepiece will not fallout
3. Place microscope at least 10 cm from the edge and the arm is facing you

Question 10

Procedure for focusing on low power (6)

Answer 10

1. Place slide on top of stage
2. Position 4x lense into optical path, click into position
3. Use coarse adjustment knob and place it all the way down
4. The move the stage all the way up
5. Use fine adjustment knob for sharp focus
6. Might need to change the diaphragm for control over the brightness

Question 11

What is the difference between the procedure of low power and high power?

Answer 11

In high power, you NEVER TOUCH THE COARSE ADJUSTMENT KNOB

Question 12

Procedure for the Wet Mount Slide (7)

Answer 12

1. Obtain the specimen
2. Place in center of the slide
3. Using dropper, place a drop of water on the specimen
4. Hold cover slip at a 90 degree angle
5. Make sure the bottom edge is in the drop of the water
6. Use probe to lower coverslip on the slide
7. Use kimwipe to dry excess water

Question 13

Why is a light microscope also called a compound microscope? (3)

Answer 13

1. Light microscope uses a combo of objective lenses
2. 3 objective lenses and the ocular
3. To achieve magnification

Question 14

Why should the specimen be centered in FV on low power before high power? (4)

Answer 14

1. Low power provides a larger FV
2. Minimum adjustments is needed
3. High power provides a smaller FV
4. Specimen completely disappear from FV

Question 15

As magnification increases, apparent size of object

Answer 15

Increase

Question 16

As magnification increases, actual size of object

Answer 16

Remains the Same

Question 17

As magnification increases, diameter of FV

Answer 17

Decrease

Question 18

As magnification increases, ability to see details

Answer 18

Increase

Question 19

As magnification increases, brightness of image

Answer 19

Decrease

Question 20

As magnification increases, distance between lense and object

Answer 20

Decrease

Question 21

How do you find out the field diameter of a different view if you already know the diameter of a lower power?

Answer 21

Magnification of LP X Diameter of LP
______________________________________
Magnification of Alternate View

Question 22

How do you find out the field WIDTH of a view if you know the field WIDTH of a different view?

Answer 22

LP Mag/HP Mag = HP FW/LP FW

Question 23

How to determine the size of object when the object(s) occupy the entire diameter

Answer 23

Diameter of FV/ Num. of objects across the diameter

Question 24

How to determine the size of object when only one object can fit in the Diameter?

Answer 24

Diameter of FV/% of occ. Diameter

Question 25

Procedure to determine what thread is on top

Answer 25

1. Start at Low Power
2. Center Slide
3. Use coarse and fine adjustment knob
4. Switch to a higher power
5. Turn fine adjustment knob toward you
6. Thread that comes into focus FIRST is on TOP
7. Thread that comes into focus LAST is on the BOTTOM

Question 26

What happens to an object when placed underneath the microscope?

Answer 26

Becomes upside down and backwards

Question 27

What is the measure of the field diameter for the 40x, 100x and 400x?

Answer 27

4000 micrometers
1600 micrometers
400 micrometers