Unit Five - Energetics - Cellular Respiration Flashcards
What is the goal of cellular respiration? (2)
- Breaks the bonds of glucose (quick energy)
- Through oxidation, releasing energy
What is the structure of ATP?
- Two adenine nitrogen bases
- Ribulose (5 - Carbon Carb.) connects to adenine to form adenosine
- Connects to 3 phosphates, forming the triphosphate
Adenine
|
Ribose
|
Phosphate - Phosphate - Phosphate
What makes ATP such an effective energy carrier? (7)
Read Through
- All phosphate groups are negative
- Forming Repulsion
- Creating the bonds to be unstable
- When high-energy bonds are broken
- The repulsion is reduced
- A significant amount of energy is released
- Energy is transferred to molecules for cell work
What are the defining differences between Autotrophs and Heterotrophs? (3/2)
Autotrophs
- Makes own food/energy
- Transforms Light Energy to Chemical Bond Energy
- Does Photosynthesis & Cellular Respiration
Heterotrophs
- Ingests Food
- Does Cellular Respiration
What is the formula for Cellular Respiration?
C 6 H 12 O 6 + 6 O 2 –> 6 CO 2 + 6 H 2 O + ATP
What are the defining differences between Photosynthesis and Cellular Respiration? (7/7)
Raw Materials, Products, Time & Where Occurs, Organelle, Type of Rxn
Photosynthesis
1. Raw Materials - Carbon Dioxide and Water
2. Products - Glucose and Oxygen
3. Occurs only in the day
4. Only occurs in organisms with chlorophyll
5. Organelle Used: Chloroplasts
6. Synthesis/Endergonic/Anabolic
7. Photophosphorolation (Makes ATP)
Cellular Respiration
1. Raw Materials - Glucose and Oxygen
2. Products - Carbon Dioxide and Water
3. Occurs Day and Night
4. Occurs in all organisms
5. Organelle: Mitochondria
6. Breakdown/Exergonic/Catabolic
7. Phosphorylation (Substrate Leveland Oxidative)
What is Aerobic Respiration? (4)
Definition, Production, Examples & Location
- Occurs in the presence of oxygen
- Produces more E (36 - 38 ATP)
- Oxidative Phosphorylation
- Mitochondria
What are 3 types of Phosphorylation? (3)
- Photophosphorolation
- Substrate Level Phosphorylation
- Oxidative Phosphorylation
What is Anaerobic Respiration? (3)
Definition, Production, Examples & Location
- Does not occur in the presence of oxygen
- Less efficient than aerobic, produces less E (2 ATP)
- Substrate Level Phopshorolayion
- ONLY occurs in the Cytoplasm (no organelles)
What is Alcohol Fermentation in Yeast & Bacteria? (4)
- Glucose is split
- CO2 released
- NADH is oxidized into NAD+
- End products: Netgain of 2 ATP, alcohol
What is Lactic Acid Fermentation in Muscle Cells of Animals? (4)
Cause, Process, Result
Cause: Lack of Oxygen causes cramps (buildup of lactic acid to slow you down, allowing muscles to survive without oxygen for a period of time
1. Glucose is split
2. NADPH is oxidized into NAP+
3. End Product - 2 ATP & Lactic Acid
What is Substrate Level Phosphorolation? (4)
Definition, Type of Process & Where it Occurs
- Phosphate from organic intermediate molecule
- Attatches to ADP to form ATP
- Anaerobic Process
- Occurs in Glycolysis & Krebs Cycle
What is Oxidative Phosphorolation? (2)
- Aerobic Process (make LOTS of ATP)
- Electron Transport Chain and ATP Synthase Complex
What is Photophosphorylation? (2)
- Light energy is converted to ATP
- Occurs in Photosynthesis
What is the process of Glycolysis? (14)
- Glucose receives a phosphate from ATP
- ATP - ADP
- Glucose receives a phosphate from ATP a second time
- ATP - ADP
- Glucose w/ 2 phosphate group on both ends split
- Forms into 2 molecules of G3P
- G3P receives an inorganic phosphate
- G3P gets Oxidized (release of e-)
- NAD+ reduces to NADH and H+ (got 2H+ from surroundings)
- Phosphate group is transferred from G3P to 2 ADP
- 2 ADP forms into 2 ATP (Substrate Level Phosphorylation)
- Pyruvate molecules forms
- Same thing occurs with the other G3P that was produced
- 4 ATP, 2 Pyruvate, 2 NADH & H+ are formed (2 ATP netgain)
Where does Glycolysis occur?
Cytoplasm - Where enzymes are located
Is NAD+ oxidized or reduced? (3)
- NAD+ accepts e- from the ETC (in glycolysis & krebs cycle) and 2 H+
- Reduced to NADH and H+
- NADH carries the e-
Is FAD+ oxidized or reduced? (3)
- FAD+ accepts e- from the ETC (krebs cycle) and 2 H+
- Reduced to NADH and H+
- FAD2 carries the e-
What is the process of the prep phase between Glycolysis and the Krebs Cycle? (11)
- Pyruvate moves thru mitochondria
- AEROBIC PROCESS
- Carbon & 2 Oxygen removed
- Released as CO2
- Leaves behind a 2C mol. (acetyl group)
- 2C group oxidized (removes H & e-)
- NAD+ receives e- and H, forms to NADH
- NADH carries e- to the ETC for ATP production
- 2 C combines w/ coenzyme A
- Forms acetyl-CoA that enters the Krebs Cycle
11 OCCURS 2X BECAUSE THERE ARE 2 PYRUVATES PRODUCED
What are the products of Glycolysis?
- 2 Net ATP
- 2 NADH + H+
- 2 Pyruvates
What are the products of Krebs Cycle?
- 2 ATP
- 6 NADH + H+
- 2 FADH2
- 4 CO2
What are the products of Prep Phase?
- 2 NADH + H+
- 2 CO2
- 2 Acetyl - CoA
Occurs 2x
What are the products of ETC?
Each NADH makes 3 ATP
Each FADH2 makes 2 ATP
Produces: 36 - 38 ATP
Why is there a range when it comes to the ATP production in the ETC? (2)
- Eukaryotes (membrane bound organelles) - 2 ATP needs to be used up for moving things across the membrane (36 ATP)
- Prokaryotes (no membrane bound organelles) - Glycolysis & Krebs cycle occurs in Cytosol, ETC occurs in the Plasma Membrane (38 ATP)
What are the steps of the Krebs cycle? (14)
- Acetyl - CoA combines with 4C
- CoA is removed
- Forms 6C
- C & O2 removed from 6c
- Releas of CO2
- H from 6C reduces NADP+ to NADPH + H+
- Steps 4 - 6 Occurs Again
- CoA (makes it reactive) and inorganic P attaches to 4C
- P removed & attaches to GDP, forming GTP
- P is removed from GTP & added to ADP
- Forms ATP
- FAD+ reduced to FADH
- NAD+ reduced to NADH + H+
- Cycles 2X due to 2 Acetyl - CoA
Where does the Krebs Cycle occur?
The Matrix of the Membrane
Where does ETC occur?
Inner Membrane of the Mitochondria
What is the ETC in Cellular Respiration? (16)
- NADH donates e- & H+ at Complex I.
- H+ and e- move down the ETC
- Integrated proteins reduce & oxidize in a cycle
- Proteins spin like a turbine
- This moves e- down the “staircase”
- Absorbs E released from the e-.
- Actively pump H+ across mem.
- FADH2 donates e- to 2nd complex
- NAD+ and FAD+ produced & return to glycolysis/Krebs cycle
- ETC sets up proton gradient
- Creates PE
- Final e- acceptor at end of the chain is O
- O combines w/ 2 H+ & forms H2O (metabolic waste)
- H+ diffuses thru ATP synthase.
- ATP synthase spins like turbine from PE
- Inorganic phosphate attaches to ADP to form ATP (Oxidative Phosphorylation)
Why is oxygen essential in the ETC? (2)
- Acts as a final e- acceptor
- W/o O2, e- would build up at the end & ETC would back up & halt
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
Final e- Acceptor
Cell Resp. - Oxygen at the end of ETC
Light Rxn - NADP+ changes to NADPH (after excited)
Calvin Cycle - PGAL is final product (takes e-)
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
Where SLP occurs
Cell Resp. - Glycolysis & Krebs
Light Rxn -
Calvin Cycle -
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
Where Oxidative Phosphorylation occur?
Cell Resp. - ETC & ATPASE
Light Rxn -
Calvin Cycle -
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
Where photophosphorylation occurs? (4)
- e- absorbs light E
- Goes thru ETC
- From Photosystem II to Photosystem I
- ETC sets up PE for ATP Synthase to form ATP
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
Common Cycle for Aerobic & Anaerobic Respiration
Glycolysis in Cellular Respirartion
What process releases Oxygen?
Photolysis in the Light Reaction
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
Purpose of Redox Reactions
Cell Resp. - Spin proteins & move e- to produce Membrane Potential in ETC
Light Rxn - Set up Membrane Potential
Calvin Cycle -
What’s the product of linear flow of e- in Photosynthesis?
- e- travel ETC
- From PII to PI
- Reducing NADP+ to NADPH
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
Purpose of ETC
Cell Resp. - Move e- to set up Membrane Potential for ATP Production
Light Rxn - Move e- to set up Membrane Potential for ATP Production
Calvin Cycle -
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
Purpose of Splitting H2O
- In Light Rxn
- Produce e- in PII
- To accelerate ETC
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
What are the e- carriers?
Cell Resp. - NADH & FADH
Light Rxn - NADP+
Calvin Cycle - NADPH
Goal of Calvin Cycle
Convert CO2 to C612O6
Goal of Krebs Cycle
- 2 Net ATP
- 6 NADH & H+ (for ETC)
- 2 FADH2
- 4 CO2
Goal of an ETC
Set up Membrane Potential/PE to synthesize ATP in ATP Synthase
(Describe the answer that aligns w/ Cell. Resp, Light Rxn & Calvin Cycle)
Cell Organelle & Location for each Rxn
Cell Resp. - Cytosol (Glycolysis), Matrix (Krebs), Inner Membrane (ETC & ATPase)
Light Rxn - Thylakoid Membrane
Calvin Cycle - Stroma
Where ATPase complexes are located?
Cell Resp. - Inner Mitochondrial Membrane
Light Rxn - Thylakoid Membrane
Purpose of Anaerobic Respiration & Types
Lactic Acid & Alcohol Fermentation
Generate ATP w/o the use of Oxygen (Survival Mechanism)
Location of Glycolysis
Cytosol
Definition of Membrane Potential (2)
- The difference in H+/charge across membrane forms GRADIENT
- That allows H+ to diffuse through ATPase