Unit 4 Part 2 - Accessioning Flashcards

1
Q

what happens before testing (specimen collection)

now called preexamination phase

A

pre-analytical phase

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2
Q

what happens during testing

now called examination phase

A

analytical phase

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3
Q

what happens after testing (results given)

now called postexamination phase

A

post-analytical

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4
Q

form used to order lab tests

A

requisiton

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5
Q

forms that serve as lab requests and report forms

A

multiple-part requisition

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6
Q

LIS

A

laboratory information system

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7
Q

technology that makes sure the correct treatment is administered to the right patient, ultimately reducing errors and ensuring patient safety

A

barcode

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8
Q

one of the most common means of transportation from getting specimens from one end of the hospital to the laboratory

A

pneumatic tube system

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9
Q

It is important to gently mix anticoagulant tubes immediately by gentle inversion __ times

A

8

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10
Q

coagulation tubes are mixed ___ times

A

3-4

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11
Q

used to diagnose meningitis infections and other brain disorders

always delivered stat

A

CSF

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12
Q

List the 3-4 tubes collected for CSF and their handling

A
  1. Chemistry/Immunology - on ice
  2. Microbiology - no ice
  3. Hematology - on ice
  4. Cytology or other - on ice
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13
Q

If one specimen is needed by 3 departments (ex. urine or CSF), it must be processed in what order?

A
  1. Microbiology - to prevent contamination
  2. Hematology - for cellular analysis
  3. Chemistry

Do not put on ice!

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14
Q

because of variability and stability of analytes, most labs use the ___ as max time limit for separating specimens as a general rule

A

two-hour limit

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15
Q

specimens should be centrifuged and separated within ___ from time of collection

A

2 hours

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16
Q

if blood is drawn in SST/PST it is stable for ___ after centrifugation

A

24 hours

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17
Q

if stored at degrees, SST/PST tube is stable for ___

A

48 hours

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18
Q

once serum has been removed from RBC, sample will be stable at RT for ___ and up to ___ at 2-4 degrees

A

RT: 8 hours

2-4 degrees: 48 hours

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19
Q

after 48 hours, serum specimens should be frozen at ___ in an aliquot tube

A

-20 degrees

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20
Q

serum specimens should be ___ before centrifugation

A

completely clotted

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21
Q

blood smears must be prepared within __ of collection

A

1 hour

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22
Q

EDTA for CBC should be tested within ___

A

6 hours

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23
Q

EDTA for CBC is stable for ___ at RT

A

24 hours

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24
Q

RNA specimens must be transported and processed ___

A

ASAP

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25
Q

RNA specimens can be stored at ___ for 48 hours

A

4 degrees

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26
Q

If not being processed within 48 hours, RNA specimens must be in aliquot tube and frozen at ___

A

-80 degrees

27
Q

urine specimens need to be protected from __ and can be tested within __ at RT

if not possible it should be refrigerated

A

light, 2 hours

28
Q

cytology specimens should be examined ASAP, if not possible use ___ to preserve the cells

A

ethanol

29
Q

a portion of specimen used for testing

prepared by transferring a portion of specimen into one or more tubes labeled with same ID info as specimen tube

A

aliquot

30
Q

serum/plasma aliquot can remain at RT no longer than __

A

8 hours

31
Q

serum/plasma aliquots that cannot be completed within 8 hours must be refrigerated at __

A

2-8 degrees C

32
Q

serum/plasma aliquots not completed within 48 hours should be frozen below ___

A

-20 degrees C

33
Q

serum/plasma should not be frozen and thawed more than ___

A

once

34
Q

little finger estimates about ___ for the aliquot tubes

A

0.5 ml

35
Q

index finger estimates about ___ for the aliquot tubes

A

1.0 ml

36
Q

middle and index finger estimates about ___ for the aliquot tubes

A

2.0 ml

37
Q

sodium fluoride tubes for glucose and EDTA tubes keep blood stable for up to ___ at RT

A

24 hours

38
Q

body temperature

A

36.4 - 37.6 C (avg 37 C)

39
Q

room temperature

A

20 - 30 C (avg 25 C)

40
Q

refrigerator temperature

A

2 - 10 C

41
Q

frozen temperature

A

-20 C (some require -70 C or lower)

42
Q

term used to describe analytes altered or destroyed by heat

A

thermolabile

43
Q

why do we chill down specimens?

A

to slow down metabolic processes

44
Q

specimens that have to be chilled should be ___ in a slurry of crushed ice (slurpee)

A

completely immersed

45
Q

Handling for:

ABG
Ammonia
Lactic acid

A

chilled

46
Q

Handling for:

Most coagulation tests
Potassium

A

must not be chilled

47
Q

Handling for:

cold agglutinins
cryofibrinogen
semen analysis

A

body temp (37 C)

48
Q

Handling for:

bilirubin
vit B12
carotene
urine specimens for poryphyrins

A

protect from light

49
Q

decrease of ___ of bilirubin is lost after ___ of light exposure

A

50%, 1 hour

50
Q

articles or substances which are capable of posing a significant risk to health, safety, property or the environment when transported by air or surface

A

dangerous goods

51
Q

3 reasons why lab personnel must be aware of TDG regulations

A
  1. transport of chemicals needed for lab
  2. transport of specimens from one site to another
  3. disposal of hazardous waste (chemical and biological)
52
Q

consists of a primary receptacle, secondary packaging, rigid outer packaging

A

triple packaging system

53
Q

blood collection tubes, urine containers or pour off tubes with leak-proof lids

A

primary package

54
Q

durable, watertight, leak-proof receptacle to enclose and protect the primary receptacles

A

secondary package

55
Q

durable rigid container, like a cooler

A

outer package

56
Q

serum specimens should be separated from whole blood before being ____

A

refrigerated

57
Q

anticoagulated venous specimens should be stored at 2-8 degrees if they will not be tested within ___

A

4 hours

58
Q

serum/plasma aliquots can remain at RT for no longer than ___

A

8 hours

59
Q

EDTA tubes are stored at RT for ___ and then discarded, frozen specimens are unacceptable

A

24 hours

60
Q

sodium fluoride/potassium oxalate tubes are stored at RT for ___

A

24 hours

61
Q

form that follows specimen from collection through to final report

has signature of everyone who has come into contact with this specimen

A

chain of custody forms

62
Q

chain of custody forms used for:

A

blood alcohol testing
urine drug testing
paternity testing
drug testing of athletes

63
Q

significantly abnormal test results, usually indicate life-threatening situations and action must be taken immediately

A

critical (panic) levels

64
Q

when phoning critical panic levels make sure that the other person ___

A

writes results down
reads it back to you