Unit 2 Quiz Flashcards
spectrophotometry
allows you to measure the absorbance and transmittance of light energy by molecules.
Absorb
When light of specific wavelength (color) is passed through a solution, some of the light energy will be absorbed.
Transmit
The remaining light energy that is not absorbed when passed through a solution, is detected and expressed on a meter as either percent transmittance (%T) or absorbance (A).
Spectrophotometer
Mode button Digital display Wavelength knob Sample holder 0% T-Knob 100% T Knob Filter switch
6 Colors of visible light
Red Orange yellow green blue violet
measured in nanometers (nm)
Red
620-750 nm
Orange
590-620 nm
Yellow
570-590 nm
Green
500-570 nm
Blue
450-500 nm
Violet
380-450 nm
Electromagnetic Radiation
Visible light is part of a large field of energy that is known as electromagnetic radiation
All energy travels through space in a pattern called
waves
smaller wavelengths=
higher energy
larger wavelengths=
lower energy