Unit 2 Quiz Flashcards

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1
Q

spectrophotometry

A

allows you to measure the absorbance and transmittance of light energy by molecules.

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2
Q

Absorb

A

When light of specific wavelength (color) is passed through a solution, some of the light energy will be absorbed.

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3
Q

Transmit

A

The remaining light energy that is not absorbed when passed through a solution, is detected and expressed on a meter as either percent transmittance (%T) or absorbance (A).

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4
Q

Spectrophotometer

A
Mode button
Digital display
Wavelength knob
Sample holder
0% T-Knob
100% T Knob
Filter switch
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5
Q

6 Colors of visible light

A
Red
Orange
yellow
green
blue
violet

measured in nanometers (nm)

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6
Q

Red

A

620-750 nm

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7
Q

Orange

A

590-620 nm

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8
Q

Yellow

A

570-590 nm

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9
Q

Green

A

500-570 nm

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10
Q

Blue

A

450-500 nm

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11
Q

Violet

A

380-450 nm

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12
Q

Electromagnetic Radiation

A

Visible light is part of a large field of energy that is known as electromagnetic radiation

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13
Q

All energy travels through space in a pattern called

A

waves

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14
Q

smaller wavelengths=

A

higher energy

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15
Q

larger wavelengths=

A

lower energy

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16
Q

If an object is red then the white light is hitting it and all the colors but red are being

A

absorbed but the red light is being transmitted.

17
Q

which color of visible light has the highest energy level?

A

violet

18
Q

Examples of electromagnetic radiation

A
microwaves
x-rays
gamma rays
UV light
TV 
radio
19
Q

A spectrophotometer can be utilized to

A

determine the concentration of a solution using a set of standards of known concentration.

20
Q

A higher concentration means

A

a larger amount of light will be absorbed.

21
Q

Visible light is only a

A

small portion of a broad spectrum of available energy called electromagnetic radiation.

22
Q

Electromagnetic Radiation travels in the form of a

A

wave, with the distance between two crests termed a wavelength.

23
Q

Visible light consists of light wavelengths from approximately

A

380-720 nanometers.

24
Q

Shorter wavelengths have

A

higher frequency and higher associated energy.

25
Q

The mode button

A

is the left button on the top and it switches between the absorption and transmittance modes.

26
Q

The Wavelength control knob

A

is the circular knob on the right side in the middle part of the spectrophotometer. The wavelength knob is used to change the wavelength.

27
Q

The 100% T Knob

A

is the circular knob on the right side of the bottom part of the spectrophotometer. It is under the wavelength knob. The 100% T knob controls how much transmittance (or brightness).

28
Q

The 0% T Knob

A

is the circular knob on the left side of the spectrophotometer on the bottom part. It is to the left of the 100% T knob. The 0% T knob is used to turn the spectrophotometer off and on, and controls the zero control.

29
Q

The Sample Holder

A

is on the left side on the middle part of the spectrophotometer. You place the test tubes in there.

30
Q

The Filter Switch

A

is on the left side on the bottom kind of underneath the spectrophotometer.

31
Q

The Digital Display

A

is on the top part of the Spectrophotometer on the left side next to the mode button. It shows the wavelength, absorbance, transmittance, and a few other things?