Chapter 6 Flashcards
All organisms are made of
cells
The cell is the
simplest collection of matter that can live.
They have the 7 qualities of life
Cell structure is correlated to
cellular function
All cells are related by their
descent from earlier cells.
Though usually too small to be seen by the unaided eye,
cells can be complex
Scientists use microscopes to
visualize cells to small to see with the naked eye
In a Light Microscope (LM),
visible light passes through a specimen and then through glass lenses
Lenses refract (bend) the light, so that
the image is magnified
Three important parameters of microscopy
Magnification
Resolution
Contrast
Magnification
the ratio of an object’s image size to its real size
Resolution
the measure of the clarity of the image, or the minimum distance of two distinguishable points.
(how clear you can see the image and magnify it)
Contrast
visible differences in parts of the sample
Light Microscopes (LMs) can magnify to about
1,000 times the size of the actual specimen
Various techniques enhance
contrast and enable cell components to be stained or labeled
Most subcellular structures, including organelles,
are too small to be resolved by a Light Microscope (LM)
Organelles
membrane-enclosed compartments
Two basic types of Electron Microscopes (EMs) that are used to study subcellular structures
Scanning Electron Microscopes (SEMs)
Transmission Electron Microscopes (TEMs)
Scanning Electron Microscopes (SEMs)
focus a beam of electrons onto the surface of a specimen, providing images that look 3-D.
-Allows you to see the surface!!
SURFACE (SSS)
Transmission Electron Microscopes (TEMs)
focus a beam of electrons through a specimen
-allows you to see the little things inside
internal ultrastructures
-Disadvantage: only dead specimen
Cell fractionation
takes cells apart and separates the major organelles from one another
-so you can study just the parts you’re interested in.
It starts out slow and short spins, then it ends up with fast and long spins
Centrifuges fractionate cells into
their component parts
Cell fractionation enables scientists to
determine the functions of organelles
Biochemistry and cytology help correlate
cell function with structure
The order the organelles fall out during Cell fractionation
- nucleus
- mitochondria and chloroplast
- microsomes
- ribosomes
The basic structural and functional unit of every organism is one of two types of cells:
prokaryotic or eukaryotic
Prokaryotic Cells
Domain Bacteria and Domain Archaea
Eukaryotic Cells
Kingdoms:
plants, animals, fungi, protists (protista)
Basic features of ALL cells
plasma membrane
semifluid substance called cytosol
chromosomes (carry genes. DNA)
ribosomes (make proteins)
Every cell needs to be able to
make proteins
Prokaryotic Cells are characterized by having
no nucleus
DNA in an unbound region called the nucleoid
no membrane-bound organelles
cytoplasm bound by the plasma membrane
Eukaryotic Cells are characterized by having
DNA in a nucleus that is bounded by a membranous nuclear envelope
membrane-bound organelles
cytoplasm in the region between the plasma membrane and nucleus
Eukaryotic cells are generally much larger than
prokaryotic cells
The plasma membrane is a
selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of every cell
The general structure of a biological membrane is a
double laye of phospholipids
Metabolic requirements set upper limits on the size of
cells
Eukaryotic cells?
The surface area to volume ratio of a cell is
critical
As the surface area increases by a factor of n^2,
the volume increases by a factor of n^3
Small cells have a greater surface area relative
to volume.
this is how eukaryotic cells can be so big
Cells need enough surface area for their
work, metabolism.
All of the membranes are put together into a eukaryotic cell and that is what
makes the surface area so big
A eukaryotic cell has internal membranes that partition the cell into
organelles
Organelles participate in
metabolism (enzymes built into their membranes)
Organelles provide
local environments for specific reactions
Plant and animal cells have most of the same
organelles
In animal cells but not plant cells
lysosomes
centrioles
flagella (in some plant sperm)
In plant cells but not animal cells
chloroplasts
central vacuole and tonoplast
cell wall
plasmodesmata
The nucleus contains most of the
DNA in a eukaryotic cell
The genetic instructions are
how a cell know what to do
Ribosomes use the information from the DNA to
make proteins
The nucleus and the ribosomes work
together in eukaryotic cells
Nucleus function
hold the DNA
Ribosomes function
make proteins
The nucleus contains
most of the cell’s genes and is usually the most conspicuous organelle
The nuclear envelope
encloses the nucleus separating it from the cytoplasm
The nuclear membrane is a
double membrane. (it folds back over)
- membrane is continuous at the pores
- pore complexes line the pore
Pores regulate the entry and exit of molecules from the
nucleus
The shape of the nucleus is maintained by the
nuclear lamina, which is composed of protein
Nucleus job
hold DNA
In the nucleus, DNA is organized into discrete units called
chromosomes
Each chromosome is composed of
a single DNA molecule associated with proteins
The DNA and proteins of chromosomes are together called
chromatin
Chromatin condenses to form discrete
chromosomes as a cell prepares to divide
The Nucleolus (not a membranous organelles) is located within the
nucleus and is the site of ribosomal RNA (rRNA) synthesis
The nucleolus is where we
put together ribosomes
The nucleolus is located within the
nucleus
the dense region
Nucleolus function
make RNA to make ribosomes
Ribosomes are particles made of
ribosomal RNA and protein
-site of protein synthesis
Ribosomes carry out protein synthesis in two locations
in the cytosol
and
on the outside of the endoplasmic reticulum or the nuclear envelope
The ribosomes that carry out protein synthesis in the cytosol are
free ribosomes-they float around free
-proteins for use in the cytosol
The ribosomes that carry out protein synthesis on the outside of the endoplasmic reticulum or the nuclear envelope are
bound ribosomes- they are physically stuck to the thing. they will have proteins that end up outside the membrane.
-proteins for membranes, packages, or secretion
Ribosomes function
make proteins (important!!!)
Endomembrane system
a series of physically connected structures
Components of the Endomembrane system
1st- Nuclear Envelope 2nd- Endoplasmic Reticulum 3rd- Golgi Apparatus 4th- Lysosomes 5th- Vacuoles 6th- Plasma Membrane
-The components go through the endomembrane system through this order
The components of the endomembrane system are either continuous or connected via transfer by
vesicles