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CC RMT AUG2024 > Triglyceride: Methods for Analysis > Flashcards

Triglyceride: Methods for Analysis Flashcards

1
Q

Triglyceride + H20 –_____-> Glycerol + 3 Fatty Acid

A

Alcoholic KOH

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2
Q

Glycerol + O2 -Periodic acid-> _____

A

Formaldehyde

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3
Q

Formaldehyde + CHROMOTROPIC acid

A

van Handel-Zilversmith

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4
Q

van Handel-Zilversmith end product

original:
CDC mod:

A

original: (+) blue
CDC mod: (+) pink

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5
Q

Formaldehyde + DIACETYL ACETONE + NH3 -> diacetyl lutidine compound

A

Hantzsch

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6
Q

Hantzsch end product

A

yellow

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7
Q

TAG + H2O -lipase-> glycerol + 3 fatty acids

Glycerol + ATP -glycerokinase-> glycerophosphate + ADP

A

enzymatic method

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8
Q

coupling enzymes:

a. glycerophosphate oxidase + ____
b. H20 absorbance at?

A

a. peroxidase
b. 500nm

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9
Q

coupling enzymes:

a. glycerophosphate dehydrogenase + ____
b. NADH absorbance at?

A

a. diaphorase
b. ↑340nm

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10
Q

coupling enzymes:

a. pyruvate kinase + _____
b. NAD absorbance at?

A

a. lactate dehydrogenase
b. ↓ 340nm

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11
Q

reference values: <150mg/dL

A

normal

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12
Q

reference values: 150-199mg/dL

A

borderline high

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13
Q

reference values: 200-499 mg/dL

A

high

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14
Q

reference values: ≥500mg/dL

A

very high

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15
Q

appearance of plasma: <200mg/dL

A

clear

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16
Q

appearance of plasma: >300mg/dL

A

hazy or turbid

17
Q

appearance of plasma: >600mg/dL

A

opaque and milky

18
Q

hyperlipoproteinemia types 1, 2b, 3, 4, 5, nephrotic syndrome, alcoholism, pancreatitis, hypothyroidism

a. hypertriglyceridemia
b. hypotriglyceridemia

A

a. hypertriglyceridemia

19
Q

malnutrition, malabsorption, hyperthyroidism

a. hypertriglyceridemia
b. hypotriglyceridemia

A

b. hypotriglyceridemia

20
Q

reference method; previously alkaline hydrolysis, solvent extraction, color reaction w/ chromotropic acid

A

GCMS

21
Q

Standing Plasma Test:

chylomicron accumulates as a _____ layer

A

floating “cream”

22
Q

Standing Plasma Test:

2mL plasma in a 10x75mm test tube is allowed to stand at

A

4degC undisturbed overnight

23
Q

Standing Plasma Test:

VLDL makes the sample remain ____ after standing

A

turbid

CC RMT AUG2024 (29 decks)

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