Triglyceride: Methods for Analysis Flashcards
Triglyceride + H20 –_____-> Glycerol + 3 Fatty Acid
Alcoholic KOH
Glycerol + O2 -Periodic acid-> _____
Formaldehyde
Formaldehyde + CHROMOTROPIC acid
van Handel-Zilversmith
van Handel-Zilversmith end product
original:
CDC mod:
original: (+) blue
CDC mod: (+) pink
Formaldehyde + DIACETYL ACETONE + NH3 -> diacetyl lutidine compound
Hantzsch
Hantzsch end product
yellow
TAG + H2O -lipase-> glycerol + 3 fatty acids
Glycerol + ATP -glycerokinase-> glycerophosphate + ADP
enzymatic method
coupling enzymes:
a. glycerophosphate oxidase + ____
b. H20 absorbance at?
a. peroxidase
b. 500nm
coupling enzymes:
a. glycerophosphate dehydrogenase + ____
b. NADH absorbance at?
a. diaphorase
b. ↑340nm
coupling enzymes:
a. pyruvate kinase + _____
b. NAD absorbance at?
a. lactate dehydrogenase
b. ↓ 340nm
reference values: <150mg/dL
normal
reference values: 150-199mg/dL
borderline high
reference values: 200-499 mg/dL
high
reference values: ≥500mg/dL
very high
appearance of plasma: <200mg/dL
clear
appearance of plasma: >300mg/dL
hazy or turbid
appearance of plasma: >600mg/dL
opaque and milky
hyperlipoproteinemia types 1, 2b, 3, 4, 5, nephrotic syndrome, alcoholism, pancreatitis, hypothyroidism
a. hypertriglyceridemia
b. hypotriglyceridemia
a. hypertriglyceridemia
malnutrition, malabsorption, hyperthyroidism
a. hypertriglyceridemia
b. hypotriglyceridemia
b. hypotriglyceridemia
reference method; previously alkaline hydrolysis, solvent extraction, color reaction w/ chromotropic acid
GCMS
Standing Plasma Test:
chylomicron accumulates as a _____ layer
floating “cream”
Standing Plasma Test:
2mL plasma in a 10x75mm test tube is allowed to stand at
4degC undisturbed overnight
Standing Plasma Test:
VLDL makes the sample remain ____ after standing
turbid
