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CC RMT AUG2024 > Laboratory Equipment and Supplies > Flashcards

Laboratory Equipment and Supplies Flashcards

1
Q

tolerates heating and sterilization for lengthy periods; subject to scratching and may cloud with strong alkali

A

borosilicate (pyrex, kimax)

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2
Q

6x stronger than borosilicate; better able to resist scratching and alkali

A

aluminosilicate (corex)

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3
Q

heat, chemical, and electrical tolerance and excellent optical properties; used for high thermal, drastic shock, and extreme chemical treatment with acids and alkali

A

high silica (vycor)

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4
Q

boron-free glass; used with strong acids and alkali

A

soft glass

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5
Q

soda lime glass; most inexpensive; releases alkali causing errors in certain pH determinations

A

flint glass

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6
Q

reduces light transmission

A

low actinic glass (amber-colored glass)

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7
Q

borosilicate, aluminosilicate, high silica

a. ↑ thermal resistance
b. ↓ thermal resistance

A

a. ↑ thermal resistance

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8
Q

soft glass, flint glass

a. ↑ thermal resistance
b. ↓ thermal resistance

A

b. ↓ thermal resistance

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9
Q

excellent temp tolerance and chemical resistance

A

teflon

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10
Q

used for pipet tips, test tubes, for cryogenic procedures (↓ temp.)

A

polypropylene

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11
Q

stronger than polypropylene; used for centrifuge tubes, graduated cylinders

A

polycarbonate

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12
Q

used for disposable transfer pipets, test tubes, bottles (binds/absorbs dyes, proteins, picric acids)

A

polyethylene

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13
Q

rigid, clear; used for test tubes, graduated tubes

A

polystyrene

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14
Q

soft, flexible but porous; frequently used as tubing

A

polyvinyl chloride

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15
Q

teflon, polypropylene, polycarbonate

a. autoclavable, ↑ thermal resistance
b. non-autoclavable, ↓ thermal resistance

A

a. autoclavable, ↑ thermal resistance

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16
Q

polyethylene, polystyrene, polyvinyl chloride

a. autoclavable, ↑ thermal resistance
b. non-autoclavable, ↓ thermal resistance

A

b. non-autoclavable, ↓ thermal resistance

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17
Q

glass pipettes:

HOLDS but does not deliver the exact volume

A

to contain

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18
Q

glass pipettes:

DELIVERS the exact volume it holds

A

to deliver

19
Q

glass pipettes:

etched ring/band near the mouth

A

blow-out pipet

20
Q

allowed to drain by GRAVITY

A

self-draining pipet

21
Q

example of blow-out pipettes with etched ring band

A

Ostwald-Folin
Serologic

22
Q

example of self-draining pipettes

A

Volumetric
Mohr

23
Q

Volumetric, Ostwald-Folin: what type?

a. transfer pipettes
b. measuring/graduated pipettes

A

a. transfer pipettes

24
Q

Serologic, Mohr: what type?

a. transfer pipettes
b. measuring/graduated pipettes

A

b. measuring/graduated pipettes

25
Q

graduated to tip

A

Serologic

(si SERologic ay GRUMADUATE)

26
Q

graduated between 2 marks/ no graduation to tip; point to point delivery

A

Mohr

(di pa grumaduate, kaya MOHR aral)

27
Q

Semi-automatic micropipettors:

uses SUCTION to draw sample into a disposable polypropylene tip; piston does not come in contact with liquid

A

air displacement

28
Q

Semi-automatic micropipettors:

  • operates like a hypodermic syringe (piston, plunger)
  • no air cushion
  • for high density and very viscous
  • used for sampling in most discrete automated systems
A

positive displacement

29
Q

Calibration for semi-automatic micropipettors:

how frequent?

A

6 mos

30
Q

Calibration for semi-automatic micropipettors:

Gravimetric method- weight of ____ delivered; most accurate

A

distilled water
(d= 1g/mL @20degC)

31
Q

Calibration for semi-automatic micropipettors:

Spectrophotometric method - absorbance of ____ and ____ delivered

A

potassium dichromate
para-nitrophenol

32
Q

Centrifuges:

tubes attain a HORIZONTAL position during spinning, vertical when at rest

A

Horizontal or swinging bucket

33
Q

Horizontal or swinging bucket rpm?

A

3,000 rpm

34
Q

Centrifuges:

tubes are at FIXED ANGLE when rotating

A

Fixed-angle or angle head

35
Q

Fixed-angle or angle head rpm?

A

7,000 rpm

36
Q

Centrifuge:

used TO SEPARATE LAYERS of different specific gravities; usually refrigerated to counter the heat produced due to friction

A

ultracentrifuge

37
Q

Ultracentrifuge rpm?

A

10,000 rpm

38
Q

Centrifuge:

for body fluid CELL COUNTS

A

cytocentrifuge

39
Q

Cytocentrifuge rpm?

A

200-2,000 rpm

40
Q

Units used to centrifuges

A

rpm
Relative Centrifugal Force (g)
Svedberg (s)

41
Q

Formula for converting rpm to RCF:

A

RCF(g) = 1.118x10^-5 x (r) in cm x rpm^2

42
Q

centrifuge QC parameters

A

timer, stopwatch
speed - tachometer/strobe light
thermometer (for refrigerated centrifuge)

43
Q

centrifuge QC frequency?

A

every 3 mos (quarterly)

CC RMT AUG2024 (29 decks)

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