Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

CC RMT AUG2024 > Methods of Glucose Measurement > Flashcards

Methods of Glucose Measurement Flashcards

1
Q

separate cells within 1hr, further delay will cause

A

false ↓

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

if delayed specimen testing use ___ to prevent glycolysis

A

sodium flouride (NaF)
SST

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

storage consideration:

↓ 7mg/dL/hour

A

Room Temperature (RT)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

storage consideration:

↓ 2mg/dL/hour

A

4degC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

hemolyzed sample will cause

A

dilutional effect = false ↓

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

effects of dextrose contamination

A

↑ plasma glucose by 500mg/dL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

reduction of cuprous iron to cuprous ion

A

Copper Reduction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Cu2+ + phosphomolybdic acid -> phosphomolybdenum

A

Folin-Wu

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Cu2+ + arsenomolybdic acid -> arsenomolybdenum

A

Nelson-Somogyi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Cu2+ + neocuproine -> Cu-neocuproine complex

A

Neocuproine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Folin-Wu and Nelson-Somogyi end product

A

blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Neocuproine end product

A

yellow-orange

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q
  • aka Hagedorn Jensen method
  • inverse colorimetry
A

Ferric Reduction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

ferricyanide + glucose -> ferrocyanide

A

Ferric Reduction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

ferricyanide color

A

yellow to orange

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

ferrocyanide color

A

colorless

17
Q

uses ortho-toluidine (aromatic amine)

A

condensation

18
Q

glucose + o-toluidine -> Schiff’s base

end product?

A

green

19
Q

H2O2 + chromogen -peroxidase-> oxidized chromogen + H2O

A

Peroxidase-coupled/Trinder Reaction

20
Q

absorbance of Peroxidase-coupled/Trinder Reaction

A

↑ A 500 nm

21
Q

measurement of the degree of oxygen consumption using a pO2 (Clark) electrode

A

polarographic method

22
Q

polarographic method requires addition of ____ and iodide or ____ ethanol

A

molybdate
iodide

23
Q

reference method for glucose determination

A

hexokinase method

24
Q

hexokinase

____ reaction is highly specific

A

coupling

25
Q

hexokinase

effect of hemolyzed/icteric sample ____ glucose level

A

falsely ↓ glucose level

26
Q

glucose + NAD -glucose dehydrogenase-> gluconolactone + NADH + H+

A

glucose dehydrogenase; UV method

27
Q

NADH + tetrazolium dye -diaphorase-> NAD + formazan blue

A

glucose dehydrogenase; c/s method

CC RMT AUG2024 (29 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions