Lipid & Lipoproteins: Methods for Analysis

Question 1

• Ultracentrifugation
• Homogenous (direct assay)
• Precipitation

Answer 1

HDL-cholesterol

Question 2

involves adjusting the sample to a density of 1.063 (potassium bromide) followed by CENTRIFUGATION AT HIGH SPEED for 2h

Answer 2

ultracentrifugation

Question 3

immunologic assay involving BLOCKAGE of non-HDL lipoproteins using an antibody to apo B-100

Answer 3

homogenous (direct assay)

Question 4

polyanion-divalent cations

Answer 4

heparin-Mn2+ precipitation

Question 5

CDC reference methods

Answer 5

ultracentrifugation
heparin-Mn2+ precipitation
Abell-Kendall

Question 6

HDL-C ref values:

<40mg/dL

Answer 6

high risk for CHD

Question 7

HDL-C ref values:

≥60mg/dL

Answer 7

low risk for CHD

Question 8

B-quantification
homogenous (direct assay)

Answer 8

LDL-cholesterol

Question 9

B-quantification involves ultracentrifugation to separate ____ and ____; and precipitation to remove ____

Answer 9

ultracentrifugation- separate VLDL, chylomicron

precipitation- removes HDL

Question 10

_____ uses _____ or other chemical to block or solubilize non-LDL lipoprotein classes to allow for quantitation of LDL

Answer 10

homogenous (direct assay)
detergent

Question 11

LDL-C formula

Answer 11

LDL= TC-(HDL+VLDL)

Question 12

Friedewald conventional method (mg/dL)

Answer 12

VLDL (mg/dL) = Triglyceride / 5

Question 13

Friedewald SI Unit (mmol/L)

Answer 13

VLDL (mmol/L) = Triglyceride / 2.175

Question 14

De Long

Conventional (mg/dL)
SI unit (mmol/L)

Answer 14

Conventional (mg/dL): VLDL = Triglyceride / 6.5
SI unit (mmol/L): VLDL = Triglyceride / 2.825

Question 15

Martin Hopkins

Conventional (mg/dL)
SI unit (mmol/L)

Answer 15

VLDL= TG/adjustable factor

Question 16

Friedewald calculation is unreliable when TG level is high

noticeable error at TG = _____
error unacceptably high at TG = _____

Answer 16

> 200mg/dL
400mg/dL

Question 17

LDL-C ref values:

<100mg/dL

Answer 17

optimal

Question 18

LDL-C ref values:

100-129mg/dL

Answer 18

near to above optimal

Question 19

LDL-C ref values:

130-159 mg/dL

Answer 19

borderline high

Question 20

LDL-C ref values:

160-189mg/dL

Answer 20

high

Question 21

LDL-C ref values:

≥190mg/dL

Answer 21

very high

Question 22

NCEP Guidelines for Acceptable Measurement Factor - CHOLESTEROL

≤ __ CV (precision)
≤ __ Bias (inaccuracy)
≤ __ Total Error

Answer 22

≤ 3% CV (precision)
≤ 3% Bias (inaccuracy)
≤ 9%Total Error

Question 23

NCEP Guidelines for Acceptable Measurement Factor - TRIGLYCERIDE

≤ __ CV (precision)
≤ __ Bias (inaccuracy)
≤ __ Total Error

Answer 23

≤ 5% CV (precision)
≤ 5% Bias (inaccuracy)
≤ 15% Total Error

Question 24

NCEP Guidelines for Acceptable Measurement Factor - LDL CHOLESTEROL

≤ __ CV (precision)
≤ __ Bias (inaccuracy)
≤ __ Total Error

Answer 24

≤ 4% CV (precision)
≤ 4% Bias (inaccuracy)
≤ 12% Total Error

Question 25

NCEP Guidelines for Acceptable Measurement Factor - HDL CHOLESTEROL

≤ __ CV (precision)
≤ __ Bias (inaccuracy)
≤ __ Total Error

Answer 25

≤ 4% CV (precision)
≤ 5% Bias (inaccuracy)
≤ 13% Total Error