Laboratory Safety Flashcards
physically remove hazard
elimination
replace hazard
substitution
isolate people from the hazard
engineering controls
change the way people work
administrative controls
protect the worker with PPE
PPE
most important means of preventing the spread of infection
handwashing
thoroughly clean between your fingers and under your fingernails for at least ___ secs
20
rinse your hands in a ___ position to prevent recontamination
downward
donning
gown - mask - goggles -gloves
doffing
gloves - goggles - gown - mask
BSC Class ___ - sterilizes only the air to be exhausted
does not protect work surface
BSC Class I
BSC Class ___ -
for microbiology
aka vertical laminar flow
sterilizes air that flows over the infectious material as well as air to be exhausted
BSC Class II
70% recirculated
30% exhauasted
A1
same as A1 but with higher intake air velocity or 100 lfm
A2
30% recirculated
70% exhausted
B1
100% exhausted
B2
BSC Class ___ -
completely enclosed with glove ports
provides highest level of personnel protection
for extremely hazardous organisms
BSC Class III
decontamination solution
5.25%NaOCl or 10% chlorine
chlorine:water dilution
1:10
wavelength of ultraviolet
<400nm
wavelength of visible spectrum
400-700nm
wavelength of infrared
> 700nm
wavelength of microwaves
3um-3mm
wavelength of low frequency
> 1cm
used for materials that give off harmful vapors
fume hood
major source of information about chemicals written by manufacturers
Safety Data Sheet (SDS)
recommended maximum allowable exposure (8hrs shift)
threshold limit value (TLV)
regulated limit enforced by OSHA
Permissible Exposure Limit (PEL)
toxicity level is ____ to TLV, PEL
inversely proportional
absorbs water when exposed to air
hygroscopic
interferes with metabolic processes
toxic substances
cancer-causing, mutation (DNA damage)
carcinogen/mutagen
causing birth defects
teratogen
Yellow diamond
reactivity/instability
White diamond
special
Blue diamond
health
Red diamond
Fire/flammability
Health 4,3,2,1,0
4- deadly
3- extreme danger
2- hazardous
1- slightly hazardous
0- normal material
Fire/Flammability flash point 4,3,2,1,0
4- below 73F
3- below 100F
2- below 200F
1- above 200F
0- will not burn
Reactivity/Instability 4,3,2,1,0
4- may detonate
3- shock and heat may detonate
2- violent chemical change
1- unstable if heated
0- stable
under special
OXY
ACID
ALK
COR
water-reactive
radioactive
actions to take in the event of fire (RACE)
Rescue
Alarm
Contain
Extinguish/evacuate
steps in operating a fire extinguisher (PASS)
Pull pin
Aim nozzle
Squeeze trigger
Sweep nozzle
Class __ ordinary combustibles (household)
A
Class __ flammable liquids
B
Class __ electrical equipment
C
Class __ flammable metals; professional firefighters
D
Class __ arsenal or materials that are liable to result in detonation
E
Class __ combustible for cooking
K