Transport

Question 1

Transport temperature for viruses

Answer 1

Refrigerator temperature (4°C) or freezing temperature (-20°C or -70°C)

Question 2

Transport of extracted nucleic acids

Answer 2

Refrigerated up to 48 hours or frozen at -70°C

Question 3

Key components of transport media for viruses

Answer 3

A. Protein - Stabilizes viral agents (stabilizer); B. Antimicrobials - Prevent bacterial or fungal overgrowth (bacteria and fungi inhibitor)

Question 4

Examples of transport media for viruses

Answer 4

Amie’s medium, Eagle’s tissue culture medium, UTM, VTM, Commercial M4 and M5, Stuart’s, Hank’s balanced salt solution (HBSS), Leibovitz-Emory medium

Question 5

BSL level for community and most non-retroviral laboratories

Answer 5

BSL 2 for routine clinical diagnosis

Question 6

BSL level for cultivation/propagation of viruses

Answer 6

BSL 2 and BSL 3

Question 7

Storage for delayed testing of specimens

Answer 7

Store in VTM at 4°C

Question 8

Sterile body fluid specimen handling before inoculation

Answer 8

Vortex before inoculation

Question 9

Handling of contaminated specimen

Answer 9

Centrifuge at 1000 x g for 15 minutes and inoculate the supernatant virus

Question 10

Bicarbonate buffer processing for culture

Answer 10

Processed with antibiotics

Question 11

Phenol red indicator filter specifications

Answer 11

Filtered with 0.22 to 0.45 µm pore size disposable filter