Fungal Specimen Collection And Hand Flashcards
Sample Processing Time for mycoviro
Process samples within 2 hours from receipt.
Refrigeration for Delayed Testing
Delay in testing of intravascular catheter tips, other medical devices (stents, surgical implants, replacement joints, etc.), lower respiratory tract or urine specimens -> Refrigerate at 4°C.
Storage of Dermatologic Specimens
Dermatologic specimens (skin, hair, nails) are very sensitive to cold → Hold at room temperature (22-25°C).
Incubation: Blood, Bone marrow, Tissue, Sterile fluids
30°C; TAT 21 days.
Incubation: Hair, Skin, Nail scrapings
30°C; TAT 21 days.
Incubation: Vaginal, Throat, Mouth samples
30°C; TAT 7 days.
General purpose/Routine media for fungi
Sabouraud Dextrose Agar (SDA)
Selective media for pathogenic fungi, inhibits saprophytic fungi (for clinically significant)
SDA with cycloheximide
Primary recovery of saprobic and pathogenic fungi
Brain-Heart Infusion Agar (BHIA), Potato flake agar, Sabouraud dextrose with brain-heart infusion (SABHI) agar
Primary recovery of pathogenic fungi not including dermatophytes (Antibiotics: chloramphenicol, cycloheximide, gentamicin)
Brain-Heart Infusion Agar (BHIA) with antibiotics, Yeast-extract phosphate agar with ammonia
Enhances recovery and sporulation of Blastomyces and Histoplasma
Yeast extract phosphate
Primary recovery of pathogenic, cycloheximide-sensitive fungi exclusive of dermatophytes
Inhibitory mold agar
Primary recovery of dermatophytes; recommended as screening medium
Dermatophyte test medium
Primary recovery of Dermatophytes (E.M.T species) = indicator phenol red
Mycosel/Mycobiotic agar
Detection of ascospores in ascosporogenous yeasts (e.g., Saccharomyces spp.) = components of yeasts - compounds of yeasts.
Acetate Ascospore Agar
Identification of Cryptococcus, Trichosporon, and Rhodotorula spp.; Separation of Trichophyton mentagrophytes from Trichophyton rubrum.
Christensen’s Urea Agar
Differentiation of Candida spp. by chlamydospore production.
Cornmeal Agar with Tween 80 and Trypan Blue
Differential identification of Aspergillus spp.
Czapek’s Agar / Czapek-Dox Agar
Identification of Cryptococcus neoformans and Cryptococcus gattii (creatinine for enhanced melanization).
Niger Seed Agar (Birdseed Agar)
Demonstration of conidia formation and the pigment production by Trichophyton rubrum; preparation of microslide cultures and sporulation of dermatophytes.
Potato Dextrose Agar
Identification of yeasts by determining fermentation.
Yeast Fermentation Broth
Identification of yeasts by determining carbohydrate assimilation.
Yeast Nitrogen-Base Agar
Enhanced recovery of Malassezia furfur.
SDA with Olive/Mineral Oil
For Trichophyton mentagrophytes.
Hair Baiting in Media