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Mycology and Virology > SPECIMEN HANDLING AND METHODS IN VIROLOGY > Flashcards

SPECIMEN HANDLING AND METHODS IN VIROLOGY Flashcards

1
Q

Vesicle collection

A

Wipe surface with sterile saline. Remove crust, unroof with sterile needle or scalpel. Fluid aspiration via capillary pipette, tuberculin syringe, or swabbing lesion base.

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2
Q

Sterile fluid handling

A

Extraction first to remove interfering proteins. False negative due to dilution.

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3
Q

Blood/Bone marrow collection

A

Disinfect skin with 70% alcohol, then iodine iodophor

For viral culture: EDTA/Heparin; for NAAT: EDTA (lavender/purple top)/AC - Acid Citrate Dextrose (white-top blue).

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4
Q

Dried blood spots

A

Capillary finger stick, air-dried for 2 hours for antibody testing and NAAT.

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5
Q

Rectal swab collection

A

Insert 3-5 cm into the anus, rotate until feces collected.

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6
Q

Feces collection

A

5-10 mL of freshly passed stool.

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7
Q

Urine collection

A

Minimum of 10 mL, centrifuge.

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8
Q

Tissue collection

A

Fresh tissue for culture; Fresh or formalinized/deparaffinized tissue for NAAT. Homogenized in VTM, centrifuged, inoculated. Debris stays in sediment.

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9
Q

Serum collection

A

Acute specimen (as soon as symptoms appear) or Convalescent specimen (2-3 weeks after acute specimen).

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10
Q

Preferred swabs

A

Dacron and Rayon swabs for most viruses. Flocked nylon swabs preferred for specimen absorption and release for fluorescent testing.

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11
Q

Antibody testing - IgM

A

Acute or ongoing infection.

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12
Q

Antibody testing - IgG

A

Past infection, immunity, or vaccination.

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13
Q

Rhinovirus virus and preferred specimen

A

Nasal

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14
Q

Influenza virus and preferred specimen

A

Throat or Nasopharynx

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15
Q

Parainfluenza virus and preferred specimen

A

Throat or Nasopharynx

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16
Q

RSV (Respiratory Syncytial Virus) virus and preferred specimen

A

Throat or Nasopharynx

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17
Q

Metapneumovirus virus and preferred specimen

A

Throat or Nasopharynx

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18
Q

SARS virus and preferred specimen

A

Throat or Nasopharynx

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19
Q

Varicella-Zoster virus and preferred specimen

A

Throat/Nasopharynx, Vesicle fluid/Scraping

20
Q

Measles virus and preferred specimen

A

Throat/Nasopharynx, Urine, Serum

21
Q

Coxsackie B virus and preferred specimen

A

Throat/Nasopharynx, Pericardial fluid

22
Q

Herpes simplex virus and preferred specimen

A

Vesicle fluid/Scraping, CSF, Brain biopsy

23
Q

Monkeypox virus and preferred specimen

A

Vesicle fluid/Scraping

24
Q

Rubella virus and preferred specimen

A

Urine/Serum

25
Q

Zika virus and preferred specimen

A

Urine/Serum

26
Q

Cytomegalovirus virus and preferred specimen

A

Urine/Serum & Blood and tissue (posttransplant)

27
Q

BK virus and preferred specimen

A

Urine

28
Q

Molluscum contagiosum virus and preferred specimen

A

Tissue

29
Q

Orf virus and preferred specimen

A

Tissue

30
Q

Warts, Human Papilloma virus and preferred specimen

A

Tissue

31
Q

Ebola virus and preferred specimen

A

Tissue, Respiratory secretion, Serum

32
Q

Marburg virus and preferred specimen

A

Tissue, Respiratory secretion, Serum

33
Q

Arbovirus and preferred specimen

A

CSF, Serum

34
Q

HIV virus and preferred specimen

A

Serum, CSF, Brain biopsy

35
Q

Polyomavirus in encephalitis and preferred specimen

A

Brain biopsy

36
Q

Rabies virus and preferred specimen

A

Corneal cell, Brain biopsy

37
Q

Parvovirus and preferred specimen

A

Serum, Amniotic fluid, Liver biopsy

38
Q

Sin nombre virus and preferred specimen

A

Serum

39
Q

LCM virus and preferred specimen

A

Serum

40
Q

Mumps virus and preferred specimen

A

Serum

41
Q

Hepatitis B virus and preferred specimen

A

Serum

42
Q

HHV-6 (Roseola infantum) virus and preferred specimen

A

Blood

43
Q

Norovirus/Norwalk virus and preferred specimen

A

Stool, Rectal swab

44
Q

Rotavirus virus and preferred specimen

A

Stool, Rectal swab

45
Q

Enterovirus and preferred specimen

A

Throat/Nasopharynx, Stool, Vesicle fluid/Scraping, Urine

46
Q

Adenovirus and preferred specimen

A

Throat or Nasopharynx, Stool, Conjunctival swab/Scraping

Mycology and Virology (16 decks)

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