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Mycology and Virology > Morphology examination > Flashcards

Morphology examination Flashcards

1
Q

CSF uses:

A

India ink, cryptococcal antigen

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2
Q

Direct examination of tissues

A

Histology stain, fluorescent antibody (selected organism)

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3
Q

Direct examination of skin, hair, nails, and other selected specimens

A

KOH/KOH with calcofluor white

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4
Q

Hyphal elements - width, hyaline vs dematiaceous; septate vs sparsely septate; special supplementary media

A

Culture - 22 to 25 deg C growth = yeast/mold

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5
Q

Culture at 30 deg C

A

Suspect dimorphic

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6
Q

Pink to salmon pigment, pellicle present in broth, pseudo or true hyphae negative

A

Rhodotorula, Custobasidium spp.

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7
Q

Pink to salmon pigment, pellicle present in broth, pseudo or true hyphae positive

A

Sporobolomyces spp.

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8
Q

Cream, buff, or white pigment, encapsulated yeast negative

A

Hyphae present

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9
Q

Cream, buff, or white pigment, encapsulated yeast positive

A

Cryptococcus

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10
Q

Hyphae present, assimilation of raffinose positive

A

Saccharomyces cerevisiae

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11
Q

Negative for raffinose, germ tube positive

A

C. albicans, dubliniensis, africana

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12
Q

Hyphae positive, arthroconidia

A

Trichosporon spp., Geotrichum spp. or related genera

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13
Q

Hyphae positive, anneloconidia

A

Saprochaete spp.

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14
Q

Spindle-shaped blastoconidia

A

Ustilago spp.

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15
Q

Negative for germ tube, collarette requires lipids for growth negative

A

Candida spp.

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16
Q

Negative for germ tube, collarette requires lipids for growth positive

A

Malassezia spp.

17
Q

Toxins (Gliotoxin, Fumagillin, Helvolic acid, Aflatoxin, Elastase, Protease, Aspartic acid proteinase, Catalase

A

Aspergillus

18
Q

Cell wall’s alpha-1,3-glucan, BAD (adhesion, immune modulator)

A

Blastomyces

19
Q

Extracellular proteinase, Capsule, Phenoloxidase (resists WBC attack), Melanin synthesis

A

Cryptococcus

20
Q

Phenoloxidase

A

Dematiaceous fungi

21
Q

Cell wall’s alpha-1,3-glucan, Intracellular growth, Histoplasma, Thermotolerance, CBP (binds calcium)

A

Histoplasma

22
Q

Estrogen-binding protein, Beta-glucan, alpha-glucan

A

Paracoccidioides

23
Q

Thermotolerance, Extracellular enzymes

A

Sporothrix

Mycology and Virology (16 decks)

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