Tissue

Question 1

Tissue procurement

Answer 1

Usually from deceased donors within 24 hrs of death (with appropriate consent)

Question 2

Allograft

Answer 2

Tissue transferred between the same species

Question 3

Autograft

Answer 3

Tissue transplanted back into the individual it was removed from

Eliminates communicable disease transmission risks

Question 4

Isograft

Answer 4

Tissue transfer between genetically identical twins

Question 5

Xenografts

Answer 5

Tissue transplant from one species to another

Question 6

Tissue processing

Answer 6

Use of aseptic techniques to remove extraneous soft tissue, cut grafts, etc

Sterilization may not be possible if sterilization would cause damage to graft function

Question 7

Osteoconductivity

Answer 7

Bone Graft act as a scaffold that allows recipient capillary growth into the graft

Question 8

Osteoinductivity

Answer 8

Bone grafts provide stimulation for new bone growth by exposing patient cells to BMPs (bone morphogenetic proteins)

Crushed bone can also be applied to bone surfaces to stimulate osteogenesis

Question 9

Matching for HLA and ABO

Answer 9

Only necessary for cryopreserved veins and arteries and frozen unprocessed bone containing marrow or RBCs

Question 10

FDA regulation

Answer 10

Institutions that provide and use tissues only within their own facility are not subject to oversight except under certain circumstances (redistribution to an affiliate at a different address)

Question 11

Retention of traceability records

Answer 11

10 years

Question 12

Autograft processing

Answer 12

Bacterial culture should be performed before processing

Should not be collected from patients with systemic infections or from an area in close proximity to an infected area

Question 13

Freezing and lyophilizing

Answer 13

Destroy cell viability