Therapeutic viruses

Question 1

therapy modifies gene that my be passed onto subsequent generation

Answer 1

germline gene therapy

Question 1

adenovirus replicates where? genome? gene transcription regulated how?

Answer 1

linear dsDNA genome replicates in nucleus with no integration

trasncription regulated with immedate early, early, and late classes of mRNA

Question 2

Retrovirus vectors

____integration,

____-term gene expression

potential for _____

replication ____

Answer 2

Retrovirus vectors

stabile integration,

long-term gene expression

potential for insertional mutagenesis

replication incompetent

Question 3

most populat site for foreign gene in vaccinia vector

Answer 3

TK gene

Question 5

therapy in which genetic modifications restircited to somatic cells with no effect on germline

Answer 5

somatic gene therapy

Question 6

strategy for gene expression with vaccinia vectors

Answer 6

1. plasmid construction with foreign gene + vaccina promoter

Question 7

adenovirus vectors

____integration,

____-term gene expression

immune response to vector ____

replication ____

Answer 7

no integration,

short-term gene expression

vigorous immune response

replication incompetent

Question 8

length of adenovirus gene expression

Answer 8

short term (does not integrate)

Question 8

has inherent tumor sleectivity for cells with activated Ras activated, wild type srain with no modifcations by recombinant DNA

Answer 8

Reovirus

Question 9

modifying ___ changes efficient binding to integrins present on many tumor cells to create more efficient binding to targets

Answer 9

RGD (arginaine, glycine, aspartic acid residue)

Question 9

in normal cells, ___ replication is restricted by activation of RNA activated protein kinase PKR

Answer 9

reovirus

Question 10

disadvantages of retrovirus vecotr

Answer 10

random integration into chromosomes > insertional mutagensis

maintaining hihg levels of gene expression

dpependence on target cell proliferation with MMLV-based vectors

Question 11

hTERT-Ad role

Answer 11

human telomerase reverse transcriptase promoter, iigh active in tumor cells but inactive in normal brain cells, allows for Adenovirus replication

Question 12

Conditionally Replicative Adenovirus, deletion of ____ or ____ regions will allow viral replication only in cells with specific dysfunctions (like p53, RB)

Answer 12

E1A or E1B

Question 16

transfer of new genetic material to the cells of an individual to benefit individual

Answer 16

gene therapy

Question 17

other Poxviruses (canarypox, Modified vaccinia ankara) do not multiply in

Answer 17

human cells

Question 18

genome, vaccinia virus

replication location

Answer 18

large, dsDNA

cytoplasmic replication

Question 19

Adenovirus genome CRAd can only replicate where ___ is already mutated (often cancer cells, impacting p53, Bcl-2)

Answer 19

ONYX-15

Question 20

creating adenovirus vector construct

Answer 20

1. delete viral genes to make room for gene of interest
2. transvect vector DNA construct into complementing cell line to produce vector particles
3. particles deliver gene to target cells, does not integrate

Question 21

preintegration complex with Vpr, integrase and matrix proteion will cross nuclear membrane in nondividing cells = ___ vector

Answer 21

lentivirus

Question 22

Retrovirus vectors retain ___ for integration and ___ for packaging

Answer 22

LTRs for integration and psi sequences for packaging