Retroviruses

Question 1

capsid core made up of structural proteins product of

Answer 1

gag gene

Question 1

Reverse transcriptase 2 polymerase functions

Answer 1

1) RNA dependent DNA pol (RNA to DNA) AND 2) DNA dependent DNA pol (second strand of DNA from first strand)

Question 1

integrase recognizes

Answer 1

sequences at ends of U3 and U5

Question 2

gp160 precursor protein cleaved to ____, must happen because gp160 cannot support membrane fusion

Answer 2

gp120 gp41

Question 3

U3 contains binding sites for ____ required for high level RNA synthesis

Answer 3

cellular transcription factors

Question 4

env protein made from

Answer 4

spliced mRNA on ER bound ribosomes

Question 5

stages of replication cycle

Answer 5

1) absorption 2) penetration and uncoating 3) reverse transcription 4) transit to nucleus 5) integration to host DNA 6) viral RNA synthesis via host pol II 6) RNA processing 7) Virion protein synthesis 8) assembly and budding 9) capsid maturation (proteolysis) (All Problem Residents Never Involve Reallystupid ResidentProgramss, PS, Are Cunts)

Question 7

differing factors complex retrovirus genome

Answer 7

mRNA for additional accessory proteins

Question 8

contents of coding region of retrovirus genome

Answer 8

Gag gene, polymerase genes, envelope gene

Question 10

genomic regions common to all retroviruses

Answer 10

R repeat at each end AND U5 AND Coding Region AND U3 region AND R region

Question 11

virion has 2 copies of ___, unique to retroviruses

Answer 11

+ssRNA genome

Question 12

integrated DNA, the ____, is longer than template RNA and has U3 and U5 duplicated at ends to form ___

Answer 12

integrated DNA, the Provirus, is longer than template RNA and has U3 and U5 duplicated at ends to form Long Terminal Repeats

Question 13

protein that becomes associated with dsDNA responsible for viral DNA integration

Answer 13

integrase

Question 14

signal for packaging found in unspliced by not spliced RNA

Answer 14

Psi signal