Lymphotropic viruses Flashcards
presentation cytomegalovirus persistent infections
atherosclerosis, immunosenescence, neuroblastoma
CMV does not encode ____ making acyclovir ineffective
thymidine kinase
HHV-6b replication in
CD4 Tcells (latency site unknown)
EBV latency II displays ___ antigens
EBNA1, LMP1, LMP2A, RNA EBER&2
HHV8 Kaposis sarcoma gamma herpesvirus replication in
CD19 B cells, endothelial cells, monocytes, keratinocytes, epithial cells
Herpesvirus 6b HHV-6b genome
dsDNA
congentialCMV permanent symptoms
hearing loss, vision loss, retardation, musculoskeletal abnormalities, seizures, septal defects
Beta herpes viruses
cytomegalovirus Herpevirus-6a, Heprevirus-6b, Herpevirus-7
viral characteristics cytomegalovirus
dsDNA, immediate, early, late stages of gene expression. Enveloped, icosahedral nucleocapsid, tegument proteins and RNA
serology of EBV, identify heterophile antibodies via
capability to agglutinate animal RBCs
CMV symptoms at birth
lesions, small size, hepatosplenomegaly, jaundice, hemolytic anemia, pneumonia, intracranial calcifications, microcephaly
CMV lytic replication in
epithelial, dendritic, fibroblast, smooth muscle, endothelial, macrophages, trophoblast
EVB mediated B cell proliferative disease activated by immunosuppression
Post transplant lymphoproliferative disorder
presentation cytomegalovirus health adults
asymptomiatic in healthy adults (mild mononucleosis)
EBV disease
infectious mononucleosis, Post transplant lymphoproliferative disorder, Lymphomas (B, T and NK cell)
presentation cytomegalovirus - birth defects
mental retardation, deafness, death
EBV lymphoma, EBV DNA in epithelial tumor cells, China, alska, tunisa and E Africa
Nasopharyngeal epithelia carinoma
HHV8 disease
Kapsosi sarcoma endothelial derived neoplasm, primary effusion lymphoma, multicentric Castleman disease
cytomegalovirus latency in
macrophages
gamma herpes viruses
Epstien Barr, Kaposi’s Sacrcoma virus
EBV latency II and III
viral antigens made in memory B cells, can lead to Burkits, Hodkin lymphoma, nasopharyngeal carcinoma
EBV mono results from rapid proliferation of
atypical T cells, (downey cells)
EBV transmission
saliva and blood, limited to epitheliam of pharynx and bcells due to restricted cellular expression
Treatment CMV
Granciclovir or valganciclovir OR Foscarnet OR Cidofovir
HHV 8 charactersitics
dsDNA, common in Africa
immune response to CMV
innate macrophage/NK/IFN response insufficient. Humoral does not clear but can limit reinfection or reactivation. Cytotoxic Tcells kill CMV infected cells (immune response cannot completely control)
EBV latency I displays ___ antigens
EBNA1 RNA EBER&2
cytomegaalovirus transmission
semen/cervical secretion/blood/milk/tears/urine/feces/saliva./transplacental
EBV lymphoma in immunosupressed and trasplant patients
Polyclonal lymphoma, lymphocytic interstitial pneumonia, Hairy oral leukoplakia of the tongue
EBV ___ tethers viral genome to host chromosome
EBNA1
EBV lymphoma, B cell lympomas
Hodkin and non-Hodgkin lymphoma
epstein barr, kaposi’s sarcoma virus latency in
plasma cells
primary infection EBV mono due to
robust expansion of Tcells, atypical or Downey Tcells
EBV latency I
antigens made in proliferating B ells, infectious mono, post transplant lymphoproliferative disorder
gamma herpes virus HHV-8 =
Kaposis sarcoma herpesvirus
EBV dissemination phase, B cells produce
heterophile antibodies (non functional)
cytomegalovirus shedding starts at
1 month post infection, can last months - 1 year
CMV infection symptoms
fever, fatigue, sore throut, liver function abnormalities, lymphocytosis, lymphadenopathy, mononucleosis (heterophile antibody negative)
EBV, loss of T cell function results in ____ diseases
b cell proliferation of disease
HHV-6b clinical disease
exanthem subitum or roseola infantum, fever, malaise, lympadenopathy, rash after fever break, no sequalae
mononucleosis in CMV infection is ___ antibody negative
antibody negative
cytomegalovirus infected cells show
owl eye, inclusion body
Ebstein Barr virus characteristics
dsDNA, nucleocapsid, tegument and envelope with glycoproteins, 3 stage gene expression
EBV lymphoma, B cell lymphoma of jaw and face, children 5-10, IgG promoter myc proto-oncogene, Associated with chromosomal translocation
Burkitt’s lymphoma
Roseolovirus (HHV-6) latency in
T-lymphocytes
diagnosis CMV
IgM testing variable, IgG avidity increases with time to define primary infection and recent vs past. Viral DNA, culture and immunofluorescence
CMB latent infection in
CD34+ hematopoeitic progenitor cells, monocytes
