Test 2- Lecture 12

Question 1

Describe the organization of DNA in the cell. What is chromatin composed of?

Answer 1

Question 2

What is the organizational component of DNA? What are the associated structures?

Answer 2

Question 3

Compare the nucleosome and chromatosome. What are the histones involved with each?

Answer 3

Question 4

What does acetylation do? HAT’s? HDAC’s?

Answer 4

Question 5

Describe the chromatin packing organization and its different sized fibers. What is it stabilized by?

Answer 5

Question 6

Summarize chromatin packing

Answer 6

Question 7

Compare euchromatin & heterochromatin. What are the 2 types of heterochromatin?

Answer 7

Question 8

What is semiconservative replication?

Answer 8

Question 9

What is a nucleoside? Nucleotide?

Answer 9

Question 10

What are the 2 types of bases? How many rings? What are the different components of DNA & RNA?

Answer 10

Question 11

What kind of bonding is important for DNA? How many hydrogen bonds do each have?

Answer 11

Question 12

What is hydrogen bonding critical for? Cytosine gets deaminated to form? Adenine gets deaminated to form?

Answer 12

Question 13

Describe the process of deamination

Answer 13

Question 14

What does each carbon in the sugar backbone have attached to it?

Answer 14

Question 15

Describe the backbone of DNA. What does the free 3’ end have? 5’?

Answer 15

Question 16

How does DNA replication begin? What does the process look like?

Answer 16

Question 17

How is DNA elongated? What direction does it proceed in? What bonds hold it together?

Answer 17

Question 18

What does the RNA primer need to bind? Which subunit is responsible for it? What is a primer replaced by?

Answer 18

Question 19

What are the DNA polymerases? What are their roles? What are the subunits?

Answer 19

Question 20

What role does the DNA polymerase have with the leading strand? Lagging strand?

Answer 20

Question 21

What does RNase H do? What follows the role of RNase H?

Answer 21

Question 22

What are the proteins associated with the replication fork? What does helicase use as energy?

Answer 22

Question 23

What do topoisomerases do? What are the classes?

Answer 23

Question 24

What do you know about Topoisomerase 1?

Answer 24

Question 25

What do you know about Topoisomerase 2?

Answer 25

Question 26

What is at the end of linear DNA molecules?

Answer 26

Question 27

What are the topoisomerases expressed in bacteria? What role do Fluoroquinolones play?

Answer 27

Question 28

What does Acyclovir used to treat? How is it incorporated into DNA?

Answer 28