Test 2- Lecture 12 Flashcards
Describe the organization of DNA in the cell. What is chromatin composed of?
What is the organizational component of DNA? What are the associated structures?
Compare the nucleosome and chromatosome. What are the histones involved with each?
What does acetylation do? HAT’s? HDAC’s?
Describe the chromatin packing organization and its different sized fibers. What is it stabilized by?
Summarize chromatin packing
Compare euchromatin & heterochromatin. What are the 2 types of heterochromatin?
What is semiconservative replication?
What is a nucleoside? Nucleotide?
What are the 2 types of bases? How many rings? What are the different components of DNA & RNA?
What kind of bonding is important for DNA? How many hydrogen bonds do each have?
What is hydrogen bonding critical for? Cytosine gets deaminated to form? Adenine gets deaminated to form?
Describe the process of deamination
What does each carbon in the sugar backbone have attached to it?
Describe the backbone of DNA. What does the free 3’ end have? 5’?
How does DNA replication begin? What does the process look like?
How is DNA elongated? What direction does it proceed in? What bonds hold it together?
What does the RNA primer need to bind? Which subunit is responsible for it? What is a primer replaced by?
What are the DNA polymerases? What are their roles? What are the subunits?
What role does the DNA polymerase have with the leading strand? Lagging strand?
What does RNase H do? What follows the role of RNase H?
What are the proteins associated with the replication fork? What does helicase use as energy?
What do topoisomerases do? What are the classes?
What do you know about Topoisomerase 1?
What do you know about Topoisomerase 2?
What is at the end of linear DNA molecules?
What are the topoisomerases expressed in bacteria? What role do Fluoroquinolones play?
What does Acyclovir used to treat? How is it incorporated into DNA?
