Lecture 15 Flashcards
What is translation?
What is the direction of translation? Where does it begin? What is the sequence/ what does it code for?
What is the genetic code table? How do you read the genetic code table? What are the 3 stop codons?
What are the characteristics of the genetic code?
What are consequences of altering the nucleotide sequence? What are the types of mutations?
What can a base pair addition/ deletion cause? What if it’s divisible by 3?
What is a trinucleotide repeat expansion? Whats an example?
What are the 3 stages of translation? What is used as energy? What all is involved?
What does a tRNA do? What is the structure of tRNA? What is codon/ anticodon pairing? Where does the amino acid attach to the tRNA? What is the sequence of the 5’ ~>3’ anticodon that pairs with AUG?
What is responsible for adding the correct amino acid onto the tRNA? How many are there? What are the 4 steps of the reaction? Does it require ATP? What do you call a charged enzyme? The enzyme has what kind of special activity?
What is the site of protein synthesis? What are the large/ small subunits for? Is there a difference between prokaryotic ribosomes and eukaryotic ribosomes? Ribosomes have what 3 binding sites for a tRNA?
What are translation factors? Are they distinguishable from prokaryotic translation factors? What kinds are there?
What are the steps in protein synthesis?
Describe the 4 steps in translation initiation in prokaryotes. What binds the start codon?
Describe the 6 steps of translation initiation in eukaryotes.
What are the 6 steps in elongation of eukaryotic translation? Are there differences between prokaryotic & eukaryotic elongation? What end do amino acids get added to? Where do peptide bonds form?
What are the sites on ribosomes? Where are the peptide bonds formed? What assists with peptide bond formation? Delivery of the next aminoacyl-tRNA is facilitated by what?
How does termination happen in eukaryotes? What recognizes the termination codons? What is the tRNA anticodon to stop codons?
What are the steps involved with mature functioning proteins?
What does Puromycin affect? What does Streptomycin affect? What does Chloramphenicol affect? What does Erythromycin affect? What does Tetracycline affect?
Central Dogma of Molecular Biology: Flow of Genetic Information
