Lecture 14 Flashcards
What is the central dogma of molecular biology? Is it the same for prokaryotes and eukaryotes?
What is transcription? What is DNA replication? How are they similar? Different?
What are the classes of RNAs? What are RNAs that only Eukaryotes possess?
What is the directionality of RNA synthesis? What is the product of RNA synthesis? What is the non-template strand referred to as?
What are the terms of transcription- +1? UTR? AUG? Upstream/ downstream? Promoter?
What are the 3 prokaryotic transcription events?
How many prokaryotic RNA polymerases are there? What are the subunits? Where does polymerase bind DNA?
What are the prokaryotic gene promoters? Where do they occur? What are the composed of?
What are the 5 steps of prokaryotic transcription?
What are the 2 ways prokaryotic transcription can be terminated? What is the sequence that’s used? Rho protein has what kind of special activity?
What does Rifampin do? What is it used to treat? What does it bind to?
What are the eukaryotic transcription events? What are the prokaryotic transcription events?
What are the types of RNA polymerases and what do they transcribe? What are GTF’s? What do they do?
What are the steps of eukaryotic transcription initiation? What is held in place by the GTF’s?
Describe the steps in eukaryotic transcription events.
What is the 5’ cap for? Why is the poly-A tail important? What’s the enzyme that puts it on? What’s the sequence?
What are the 5 steps of RNA splicing? What directs the removal of an intron? What ligates the exons together?
What is β-thalassemia? The production of what is defective? What’s the cause?
What is alternative splicing? What is the benefit of it?
What is mRNA editing? What is an example of it?
What are the post-transcriptional processing sequence of events? When does it happen?
What determines the direction of transcription?
Compare and contrast prokaryotic and eukaryotic transcription. What is the genome organization? What does the transcription complex consist of? What is the promoter recognized by? What are the start sequences? What is the RNA processing? What is the RNA polymerase types?
