TCA Cycle- Lecture 9/17/21

Question 1

Pyruvate dehydrogenase

Answer 1

Turns pyruvate to acetyl CoA giving off 2 NADH

Question 2

Energy Product of TCA

Answer 2

~38 ATPs

Question 3

Coenzymes of Pyruvate dehydrogenase

Answer 3

Thiamine, Lipoate, FAD, NAD+

Question 4

Coenzyme A

Answer 4

Long complicated model, active chemically at thioester

Question 5

Thiamine pyrophasphate

Answer 5

Coenzyme that participates in decarboxylation

Question 6

Lipoate

Answer 6

Coenzyme of pyruvate dehydrogenase that gets reduced in reaction, needs FAD to oxidize it

Question 7

FAD role in pyruvate dehydrogenase

Answer 7

Riboflavin, oxidizes Lipoate, gets oxidized by NAD+

Question 8

Product inhibition of PDH

Answer 8

Acetyl CoA and NADH directly inhibit PDH, sign the cell is energetically good

Question 9

Kinase inhibition of PDH

Answer 9

When PDH is phosphorylated, it is inactivated. PDH kinase activated by acetyl coA and NADH and inhibited by CoA, pyruvate and NAD+

Question 10

PDH regulation by phosphatase

Answer 10

Phosphatase activates PDH, activated by Insulin and Ca2++