Tal's notes, mid term 1

Question 1

What receptors are used for the regulation of NK cells activity?

Answer 1

Killer Activetde Receptor

Killer Inhibited Receptor

Question 2

T cell receptor structure

Answer 2

Membrane bound
a and b chains (varieble and constant regions)
attached carbohydrates

Question 3

TCR is part of the T receptor complex, including-

Answer 3

CD3 and zeta chains

Question 4

amino terminal variable region of Ig molecule consist of ______ AA (number)

Answer 4

100-110

Question 5

________ resolves Ab molecule into 3 parts:
1.
2.
3.

Answer 5

Papain cleavage

1. Fab region
2. Fab region
3. Fc region

Question 6

Name of the light chain sequence that interacts with the bound Ag

Answer 6

CDR
Complementarity Determining Regions
(CDR1/2/3)X4

Question 7

which Ig is in the highest amount in the serum

Answer 7

IgG

then (A>M>D>E)

Question 8

IgM acts as the

Answer 8

Main Ab of primary response

Question 9

IgG acts as the

Answer 9

Main blood Ab of seconday response

Question 10

IgE acts as

Answer 10

Ab of allergy and anti-parasitic activity

Question 11

What does Idiotype means?

Answer 11

Unigue AA sequence of the Variable region of a given Ab (epotope specificity)

Question 12

Light chain V-region constructed from __ gene segments:

Answer 12

2

1. V
2. J

Question 13

Heavy chain V-region constructed from __ gene segments

Answer 13

3

1. V
2. J
3. D

Question 14

On which chromosome can we find Ig heavy chain gene

Answer 14

14

Question 15

which TCR gene can we find on chromosome 7?

Answer 15

B

Gamma

Question 16

which TCR gene can we find on chromosome 14?

Answer 16

a

Delta

Question 17

On which chromosome can we find Ig k light chain gene

Answer 17

2

Question 18

On which chromosome can we find Ig lamda light chain gene

Answer 18

22

Question 19

Which sequence ensure that one of each V D J / V J will apear?

Answer 19

RSS

Recombination Signal Sequence

Question 20

Which enzyme catalyses DNA breakage

Answer 20

RAG Endonuclease (1/2)

Question 21

Which enzyme adds N-region nucleotides?

Answer 21

TdT

Terminal deoxynucleotidyl transferase

Question 22

What enzymes are called togather VDJ recombinase enzyme complex?

Answer 22

TdT
Artemis
RAG