Support tissue Flashcards

1
Q

What are the types of connective tissue?

A
  1. Ordinary
  2. Supporting
  3. Vascular
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2
Q

How is ordinary tissue classified

A
  1. Loose CT - fibres create a loose open framework (adipose, areolar, reticular)
  2. Dense CT - Fibres densely packed (Elastic, dense regular, dense irregular)
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3
Q

How is supporting CT classified

A
  1. Cartilage - Forms solid rubbery matrix (hyaline, elastic, fibrocartilage)
  2. Bone - forms solid crystalline matrix (compact, spongy)
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4
Q

How is vascular CT classified?

A
  1. Blood - Contained in cardiovascular system

2. Lymph - Contained in lymphatic system

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5
Q

What are the 3 germinal layers in the embryo

A
  1. Endoderm (inner)
  2. Mesoderm - where connective tissue is derived from, from mesenchyme tissue
  3. Ectoderm (outer)
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6
Q

What is the major type of cell population in connective tissue?

A

FIBROBLAST and FIBROCYTES = most important cell

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7
Q

How do you identify fibroblasts, where locates and what responsible?

A
  1. well distributed in CT
  2. responsible for active production of extracellular matrix, fibres adn ground substance:
  3. Large centrally located nucelus
  4. granular appearance
  5. pointing cytoplasm
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8
Q

When the fobroblast has done it job how do you identify

A

Now called fibrocyte

1. Looks like: nucleus condensed, small darkly staining, no granular structure

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9
Q

How do you identify a mature fibroblast from a fibrocyte

A
  1. Fibroblast = actively produce extracellular matrix = ground substance and fibres. Prominent nucleoli which is elongated, basophilic cytoplasm, lighter stain
  2. Fibrocyte: resting phase = condensed, elongated in direction of collagen fibres, fine processes, difficult to see with LM, involved in the maintenance of the ECM, can differentiate into other types of CT cell, darkly staining
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10
Q

Ordinary connective tissue: Loose areolar

A
  1. Fibroblasts everywhere
  2. Plasma cells, mast cells, macrophages
  3. Collagen, elastic, reticular fibres
  4. Blood vessels
  5. e.g. under skin: epidermal layer = dermis connective tissue layer, lining of GI, resp and urinary tract
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11
Q

Histologically what does collagen tissue look like

A

lightly stained, surrounds fibroblasts and cytes, swooshy appearance

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12
Q

Ordinary connective tissue: loose adipose

A
  1. Function: reserve for food, act as fuel, insulate against heat loss, supports and protects organs
  2. cell type = adipocyte
  3. fat stores in large vacuole so nucleus is pushed to boundary
  4. appear clear empty cells as fat dissolved when processing = nothing to stain.
  5. Chicken wire
  6. each adipocyte is supported by a delicate network of collagen and reticular fibres
  7. few fibrocytes, mast cells, sparse amorphous ground substance
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13
Q

How to differentiate between brown and white fat

A
  1. white = one big vacuole
  2. Brown = many “droplets” within a circle
  3. Brown = nucleus can sometimes be inc centre, white nucleus os always pushed to periphery
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14
Q

Ordinary connective tissue: loose reticular

A
  1. Around BV
  2. hard to discriminate as finer fibres
  3. function = filter fluid, cells can’t pass through
  4. stained by silver impregnation
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15
Q

Ordinary connective tissue, dense irregular

A
  1. lots and lots of collagen fibres = dense
  2. Collagen fibre no clear arrangement = hip hazard! swirly
  3. reticulum layer in dermis of skin
  4. fibroblasts, BV, immune cells, collagen fibres
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16
Q

Ordinary connective tissue: dense regular

A
  1. fibres regularly arranged in longitudinal bundles (collagen or elastic) in parallel
  2. Specific sites e.g. tendons, ligaments
  3. in between muscle fibres = CT component endomesium
  4. bundles = thick
  5. fibrocytes sit in direction of muscle fibres
17
Q

Ordinary connective tissue: Dense Elastic

A

Elastic connective tissue

  1. squiggly lines
  2. bladder, lungs, aorta: expand and recoil
  3. Protein in elastic fibre = elastin
18
Q

What is liquid/ fluid connective tissue

A
  1. blood: plasma and lymph

2. Cells circulate in a liquid extracellular matric

19
Q

Explain the role of connective tissue in the basement membrane

A
  1. allows cell adhesion - anchoring junction: hemidesmosomes
  2. diffusion barrier: epi lining is deprived of blood circulation so O2 and nutrients diffuse from underlying CT to absement membrane to underlying cells
  3. Regulation of cell growth: interface between epi lining adn CT = limits epi growth
20
Q

Supporting tissue: Hyaline Cartilage

A
  1. chondrocyte = contained in a lacuna = dormant and responsible for maintenance
  2. chondroblast = produces ECM = large nuclei = darker well rounded cell
21
Q

Supporting tissue: Fibro Cartilage

A
  1. fibrous and cartilagenous connective tissue

2. Function = flexibility and hardness e.g. annulus fibrosus of intervertebral disk of back bone : inner bit

22
Q

Supporting tissue: Elastic cartialge

A
  1. recoil
  2. elastic fibres along with chondrocyte and blasts
  3. e.g. ears hard but can move