Stem Cells

Question 1

iPS cells are ______.

Answer 1

Induced pluripotent stem

Question 2

Treatment for Epidermolysis Bullosa

Answer 2

• Allogenic bone marrow transplant (reduction of symptoms).

Question 3

Describe the steps for MSC homing into the bone marrow

Answer 3

Mesenchymal Stem Cell homing with the flow of blood–>

1a. Tethering, 1b. rolling ,
2. activation,
3. firm adhesion,
4. transmigration,
5. chemotaxis & parenchymal lodgment.

Question 4

Factors to revert Somatic cells to ES-like Stem cells.

Answer 4

Four factors: Oct3/4, Sox2, C-Myc, Klf4

Question 5

Clincal presentations of RDEB.

Answer 5

• If they live into young adulthood they will succumb to aggressive skin cancers.
• Blistering and development of scar tissues that can eventually turn the hands/feet into “clubs” (fused digits).

Question 6

Mutation of RDEB

Answer 6

• mutated in the COL7A1 gene.

- mutated Type 7 collagen.

Question 7

Genome Editing Technologies

Answer 7

• Zinc Finger
• Talen
• CRSPR

Question 8

Requirements for clinically relevant gene editing.

Answer 8

1. Non-viral methods for delivering gene-editing components.
2. Foot-print free: avoid the use of antibiotic-hesitance cassettes for selection of corrected clones.
3. Genetically safe: avoid off-target events.