Histology

Question 1

Describe the appearance of connective tissue under microscope.

Answer 1

Typically white unstained portion of the slide with groups of disorganized nuclei.

Question 2

4 extracellular components around the connective tissue.

Answer 2

GAG’s, Collagen (stained), lymphocytes, Adipocytes,

Question 3

Describe Simple Columnar Epithelial cells.

Answer 3

uni-layered, column shaped cells that line

Question 4

Describe lymphocytes under microscope.

Answer 4

Very round, dark nuclei.

Question 5

Describe fibroblasts under microscope.

Answer 5

Flat, thin, elongated nucleus typically in the connective tissue compartment.

Question 6

Describe the functions & roles of fibroblast cells.

Answer 6

Secretes extracellular matrix material including collagen, matrix metallo-proteins.

Question 7

Describe major differences between connective tissue of skin and that of small intestine lamina propria.

Answer 7

• The skin has lower cell density relative to the ECM than in the small intestine.
• small intestine

Question 8

lymphocytes and white blood cells in the small intestine.

Answer 8

• White blood cells are the “immigrants” of the small intestine.
• Small intestine has more lymphocytes for immune defense.

Question 9

Describe cartilage under microscope.

Answer 9

• Cartilage has long chains of cells due to “interstitial growth”. This is located on the epiphyseal growth plate.
• The cells embedded in the cartilage will always be chondrocytes. Cells in this region but close to the bone are a bit more differentiated and do not really divide.

Question 10

What is occurring in the bone matrix?

Answer 10

• Chondrocyte hypertrophy and calcification of the matrix

- There is a battle between chondrocytes and osteoclast to degrade and build the bone.

Question 11

Describe the osteocyte in bone.

Answer 11

-Osteocyte is embedded in the bone matrix, it’s precursor is the osteoblast.

Question 12

Describe Bone Marrow under microscope.

Answer 12

-identified as densely packed nucleated cells with in a pocket in the bone.

Question 13

What is bone marrow?

Answer 13

-Red bone marrow is hematopoietic stem cells. So called because it stains red.

Question 14

What cranks out the cell matrix? what is it’s progenitor?

Answer 14

-Osteoblast (NOT osteocytes).

• The precursor is the osteoprogenitor
• typically not in the bone matrix.

Question 15

Describe bone under microscope.

Answer 15

-Bone stains dark and is not nucleated. It does have cells embedded in it that are nucleated themselves.

Question 16

What is the appearance of adipose cells?

Answer 16

• White, bright blobby.

- The nuclei of adipose cells are pushed against the cell membrane by the accumulation of fat.

Question 17

Describe appearance and location of Collagen

Answer 17

Located in the connective tissue, it is slightly stained, in a region of tissue that appears disorganized.

Question 18

Describe the appearance of skeletal muscle.

Question 19

Describe the function and location basal lamina.

Answer 19

• Functions:
  1. Basal lamina separates epithelial cells to underlying connective tissue.
  2. Selective permeability of cellular movement from one layer to the next.
  3. it has molecules in it that contribute to the basal polarity.

-Location: It is very tightly bound to the basal side of epithelial cells.

Question 20

Describe simple squamous cells.

Answer 20

-simple squamous epithelium is a single layer of flat cells in contact with the basal lamina